大球盖菇提取物对CCl4所致肝损伤小鼠的抗氧化作用研究

食品科学 ›› 2008, Vol. 29 ›› Issue (12): 663-667.

大球盖菇提取物对CCl4所致肝损伤小鼠的抗氧化作用研究

王晓炜, 王峰, 陶明煊, 程光宇, 奚清丽, 吕中明

南京师范大学金陵女子学院；南京师范大学生命科学学院；江苏吴中大自然生物工程有限责任公司；江苏省疾病预防控制中心；

出版日期:2008-12-15 发布日期:2011-12-08

Antioxidation of Stropharia rugoso-annulata Extract（SRE） on CC14-induced Acute Liver Injury in Mice

WANG Xiao-Wei, WANG Feng, TAO Ming-Xuan, CHENG Guang-Yu, XI Qing-Li, 吕Zhong-Ming

1.Ginling College,Nanjing Normal University,Nanjing 21097,China;2.School of Life Science,Nanjing Normal University,Nanjing 210097,China;3.Jiangsu Wuzhong Nature Biotech Co. Ltd.,Nanjing 210097,China; 4.Jiangsu Centers for Diseases Prevention and Control,Nanjing 210093,China

Online:2008-12-15 Published:2011-12-08

摘要/Abstract

摘要： 目的:研究大球盖菇提取物(SRE)对小鼠肝和主要脏器组织的抗氧化作用。方法:小鼠连续30d给予低(100mg/kg·d)、中(200mg/kg·d)、高(400mg/kg·d)三个剂量的SRE后,灌胃给予CCl4(80mg/kg·d)建立动物急性肝损伤模型,动物处死后取血液和脏器测定各项抗氧化指标。结果:高剂量S R E能降低血清A L P活性,提高肝总S O D、Mn-SOD、Cu,Zn-SOD及GSH-Px活性,降低肝组织MDA含量,与模型组相比有显著性差异(p<0.01),且三个剂量呈现了一定的量效关系。主要脏器的抗氧化指标测定表明,SRE能显著提高心和肾SOD和GSH-Px活性(p<0.01),显著降低心组织MDA含量(p<0.01);与对照组相比,模型组脾总SOD和Mn-SOD表现出代偿性增高(p<0.01),给予SRE后可使升高的SOD活性恢复到对照组水平。分析表明SRE的主要抗氧化成分为多糖和黄酮。结论:SRE对CCl4所致急性肝损伤小鼠的肝和主要脏器的氧化损伤具有较好的保护作用。

关键词: 大球盖菇, 提取物, 急性肝损伤, 脏器, 抗氧化作用

Abstract: Objective:This study was designed to evaluated the antioxidant effects of SRE on liver and main organs in mice with CCl4-induced acute liver injury. Methodes:The CIR mice were administered by gavage with SRE(100,200 and 400 mg/kg·d,respectively) for 30 days before the administration with a dose ofCCl4(80 mg/kg·d). The mice were sacrificed at 24 h after administration with SRE and their blood,liver,heart,kidney and spleen were collected for assaying antioxidant parameters,respectively. Results:The 400 mg/kg·d dose of SRE significantly(p<0.01) lowers the serum ALT,while the hepatic activities of total SOD,Mn-SOD,Cu,Zn-SOD and GSH-Px are significantly increased(p<0.01),and content of MDA is significantly(p<0.01) lowered when compared with CCl4 administration group. SRE could significantly increase the activities of SOD and GSH-Px in heart and kidney,and lower the content of MDA in heart,compared with CCl4 administration group. The activities of total SOD and Mn-SOD of CCl4 administration group are compensatorily increased in spleen compared with the normal group(p<0.05). After the SRE administration,these indexes are decreased to the normal levels. The component analysis of SRE indicated that polysaccharides and flavonoids may be main antioxidant components. Conclusions:SRE can be proposed to protect the liver and various tissues against CCl4-induced oxidative damage in mice.

Key words: Stropharia rugoso-annulata, extract, acute liver injury, organ, antioxidation

王晓炜, 王峰, 陶明煊, 程光宇, 奚清丽, 吕中明. 大球盖菇提取物对CCl4所致肝损伤小鼠的抗氧化作用研究[J]. 食品科学, 2008, 29(12): 663-667.

WANG Xiao-Wei, WANG Feng, TAO Ming-Xuan, CHENG Guang-Yu, XI Qing-Li, 吕Zhong-Ming . Antioxidation of Stropharia rugoso-annulata Extract（SRE） on CC14-induced Acute Liver Injury in Mice[J]. FOOD SCIENCE, 2008, 29(12): 663-667.

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