食品科学 ›› 2007, Vol. 28 ›› Issue (2): 44-47.

• 基础研究 • 上一篇    下一篇

芝麻饼粕中抗氧化成分的提取及其活性研究

 董英, 高音   

  1. 江苏大学生物与环境工程学院; 江苏大学生物与环境工程学院 江苏镇江212013; 江苏镇江212013;
  • 出版日期:2007-02-15 发布日期:2011-12-31

Extraction of Antioxidant from Sesame Cake and Its Activity Study

 DONG  Ying, GAO  Yin   

  1. College of Biology and Environmental Engineering, Jiangsu University, Zhenjiang 212013, China
  • Online:2007-02-15 Published:2011-12-31

摘要: 研究了芝麻木脂素的提取工艺及其抗氧化性能。以料液比、浸提时间、温度、提取次数为考察因素,采用单因素试验及正交试验对芝麻饼粕中木脂素进行了提取条件优化的试验。试验结果表明,料液比1:6,浸提时间10h,温度55℃及提取次数3次为最佳提取条件,芝麻木脂素粗品的提取率为2.62%。用分光光度法测定了芝麻饼粕提取物对DPPH·的清除作用和对小鼠离体组织匀浆脂质过氧化及Fe2+-VC体系诱导的脂质过氧化的抑制作用。结果发现芝麻饼粕提取物具有良好的抑制脂质过氧化的作用。

关键词: 芝麻, 饼粕, 提取物, 抗氧化活性

Abstract: The extraction technology and antioxidant activity of lignans in sesame cake were studied. Solid-liquid ratio, extraction time, temperature, times were selected as four factors to design the orthogonal test, and the optimum conditions of extraction were obtained. The results showed that the optimum conditions for the extraction are as follows: Solid-liquid ratio 1:6, extraction time 10h, temperature 55℃ and times 3. The crude extract rate was accounted for 2.62%. The antilipid-peroxidationof the extract on liver was assayed by the determination of malondialdehyde(MDA) with TBA spectrometric method. The results showed that the extract of sesame cake can inhibit the lipidperoxidation of liver and the lipidperoxidation of mitochondria can be induced by Fe2+-VC.

Key words: sesame, cake, extract, antioxidant activity