食品科学 ›› 2003, Vol. 24 ›› Issue (5): 79-81.

• 工艺技术 • 上一篇    下一篇

法夫酵母原生质体紫外线诱变育种的研究

 卫军, 陈春涛, 王岁楼, 彭新榜, 王军   

  1. 郑州轻工业学院食品与生物工程系
  • 出版日期:2003-05-15 发布日期:2011-12-13

Study on Ultraviolet Mutation Breeding of Protoplast of Phaffia rhodozyna

 WEI  Jun, CHEN  Chun-Tao, WANG  Sui-Lou, PENG  Xin-Bang, WANG  Jun   

  • Online:2003-05-15 Published:2011-12-13

摘要: 以法夫酵母(Phaffia rhodozyma)为出发菌株,采用紫外线对其原生质体进行诱变育种,筛选出一株最适生长温度比诱变前提高10℃的突变菌株,该菌株β-胡萝卜素产量比突变前提高了121%。经多次传代生产性能稳定。

关键词: &beta, -胡萝卜素, 原生质体诱变, 发酵

Abstract: In this experiment, the natural pigment of Vaccinium vitis-idaea linn has been purified, and its spectrum characterisicdetermination was described afterward. The effects caused by temperature, illumination and pH on the stability of Vacciniumvitis-idaea linn pigment and the reducing quality of the pigment have been studied. Food addiitives added into as enhancers, havebeen studied emphatically. Orthogonal experimental design was put up with four food addiitives having better stabilizingeffect. The optimum stable conditions were : the concentration of succinic acid, trans-ferulic acid, para-hydroxybenzoic acidand naringin was respectively 0.09%, 0.03%, 0.08% and 0.01%.

Key words: Vaccinum vitis-idaea linn, natural pigment, stability, orthogonal experimental design