食品科学 ›› 2000, Vol. 21 ›› Issue (8): 18-22.

• 基础研究 • 上一篇    下一篇

唾液链球菌嗜热亚种LCX2001胞外多糖合成条件的研究

顾瑞霞, 刘爱萍, 程涛, 骆承庠   

  1. 扬州大学农学院食品科学系; 东北农业大学畜产品加工研究所
  • 出版日期:2000-08-15 发布日期:2011-12-01

Study on Exopolysaccharide Synthesis by Streptococcus salivarius subsp. thermophilus LCX2001 Grown in Chemically defined Medium

 GU  Rui-Xia, LIU  Ai-Ping, CHENG  Tao, LUO  Cheng-Xiang   

  • Online:2000-08-15 Published:2011-12-01

摘要: 从众多的用于乳酸菌生长的合成和半合成培养基(MRS、M17、ATP、SL、Elliker、SDM),选择了ATP作为唾液链球菌嗜热亚种 LCX2001胞外多糖产生的基本培养基,在此基础上开发了 LCX合成培养基,并对碳源、培养基的初始pH、发酵温度和发酵时间进行了探讨。该菌生物合成EPS的工艺条件为:葡萄糖和/或乳精是该菌株合成EPS的合适的碳源,添加量为20g/L,培养基的初始pH值为6.5,发酵温度为35℃,发酵时间为20h。

关键词: 胞外多糖, 培养基, 生物合成, 乳酸菌

Abstract: Among the mediums MRS, M17, ATP, SL, Elliker, SDM used for lactic acid bacterium growth, a new culture medium ATP was chosen for the synthesis of exopolysaccharide (EPS) by Streptococcus salivarius subsp. thermophilus LCX2001. When grown in a chemically defined medium with glucose and/or lactose as the substrate carbohydrate added in the amount of 20g/L. The production of EPS increased at higher temperatures, but the bacterium rapidly lost its EPS producing cap ability at 47℃, while the optimization temperature was 35℃,initial pH of medium was 6.5, and the fermentafion time was 20h.

Key words: Exopolysaccharide Medium Synthesis Lactic Acid Bacteria