食品科学 ›› 2007, Vol. 28 ›› Issue (5): 59-62.

• 基础研究 • 上一篇    下一篇

Candida utilis提取液的组成对D001阳离子交换树脂分离谷胱甘肽效率的影响

 王淼, 苏晓晋   

  1. 江南大学食品学院食品科学与安全教育部重点实验室; 江南大学食品学院食品科学与安全教育部重点实验室 江苏无锡214036; 江苏无锡214036;
  • 出版日期:2007-05-15 发布日期:2011-12-31

Effects of Candida utilis Extract Compositions on Glutathione Seperation Efficiency of D001 Cation Ion Exchange Resin Columns

 WANG  Miao, SU  Xiao-Jin   

  1. Key Laboratory of Food Science and Safety,Ministry of Education,School of Food Science and Technology,Southern Yangtze University,Wuxi 214036,China
  • Online:2007-05-15 Published:2011-12-31

摘要: 研究富含谷胱甘肽(GSH)的Candida utilis WSH 02-08提取液的组成对其在D001阳离子交换树脂上交换效率的影响。结果表明,提取液中大分子肽对GSH在D001阳离子交换树脂上交换效率有较大影响。采用超滤处理酵母提取液和改进从酵母中提取GSH的方法可以大大降低样品中大分子肽的含量,相对分子质量分别降低到小于2000和4000,D001阳离子交换树脂对其中GSH的吸附性能明显改善,吸附效率从原来的40%左右提高到70%以上。

关键词: 谷胱甘肽, 离子交换, 超滤

Abstract: In this paper,the effects of the Candida utilis WSH 02-08 extract compositions on the glutathione(GSH)adsorption efficiency of D001 cation ion exchange resin columns were studied.The results showed that the big molecular weight polypeptides obviously affects the adsorption efficiency of D001 cation ion exchange resin for GSH.By ultrafiltraion treatment of the extract with respect to a suitable extract method,the content of the big molecular weight polypeptides in sample decreases largely,down to MW<2000 and MW<4000 respectively,whereas the adsorption efficiency of D001 cation ion exchange resin for GSH increases obviously from about 40% to above 70%.

Key words: glutathione, ion exchange, ultrafiltraion