食品科学 ›› 1998, Vol. 19 ›› Issue (4): 10-12.

• 食品科学 • 上一篇    下一篇

高压下牛乳IgG的变性及稳定化作用

 张和平, 德力格尔桑, 郭军, 母智深; 张玉成, 祝恒明   

  1. 内蒙古农牧学院食品工程系; 兵器工业部五十二研究所
  • 出版日期:1998-04-15 发布日期:2011-12-10

Denaturation of Bovine milk IgG at High pressure and its stabilization

 He-Ping, DE  Li-Ge-尔Sang, GUO  Jun, MU  Zhi-Shen-; Zhang-Yu-Cheng, ZHU  Heng-Ming   

  • Online:1998-04-15 Published:2011-12-10

摘要: 高压下(200~700MPa)牛乳IgG的变性及稳定化研究表明:随着蔗糖浓度的提高,IgG在高压下的稳定性增大,并伴随IgG最大发射波长的蓝移和荧光强度的降低。蔗糖对IgG在高压下的稳定化作用是由于其增加了IgG分子内部疏水交互作用,从而稳定了其三级结构。

关键词: 牛乳免疫球蛋白, 高压, 蔗糖, 变性

Abstract: The denaturation of bovine milk IgG at high pressure (200 -700MPa) and its stabilization were investigated. With the increase of sucrose concentration, the stability of IgG was increased. In accompany with this result, marked blue shift of maximum emission wavelength of IgG was observed, and fluorescence intersity of IgG was decreased. Increased hydrophobic interactions inside IgG molecule in presence of sucrose was considered to be responsible for its stabilization.

Key words: Bovine Milk Immunoglobulin High pressure Sucnose Denaturation