关键词: 杭白菊, 总黄酮, 总酚, 自由基清除剂

Abstract: Six species chrysanthemum samples,including 4 Hangbaiju,1 Huangju and 1 Yeju,were collected from the famous“ Hangbaiju Kingdom of China",Tongxiang city,Zhejiang province.Fresh flower was dried by microwave oven assisted by hot air circulation,and extracted individually by thermal reflux with 70％ ethanol－ water solution.The contents of total phenols （TP） and total flavonoids （TF） of these extracts were measured by spectrophotometric analysis.Obvious differences were found in these chrysanthemums:Yizhongdabaiju had the highest contents at 9.76％ TP and 4.46％ TF （in dry base of flower）,and Yeju was the lowest one at 3.21％ TPand 1.99％ TF.Chemiluminescence method demonstrated that all of samples had good scavenging capacity on reactive oxygen free radicals.The IC 50 ranged in 0.54～ 1.98mg/ml to· OH and 24.2～ 54.5μ g/ml to O2·－ ,respectively.Among these chrysanthemums,Dayangju had the strongest scavenging activity to O2·－ ,and Ruangengxiaoyangju was the weakest one;similarly,Zaoxiaoyangju had the strongest scavenging activity to· OH and Yizhongdabaiju was the weakest one.This research showed no obvious correlativity between the phenolic compound content of chrysanthemums and their anti－ free radical capacity.

Key words: Chrysanthemum Hangbaiju Total flavonoid Total phenols Free radical scavenger