响应面法优化酵母多糖的提取工艺

doi:10.7506/spkx1002-6630-201224019

食品科学 ›› 2012, Vol. 33 ›› Issue (24): 92-96.doi: 10.7506/spkx1002-6630-201224019

响应面法优化酵母多糖的提取工艺

王慧1,2，程富胜3，罗永江3，董鹏程3，张霞1,*

1.甘肃农业大学生命科学技术学院，甘肃兰州 730070；2.甘肃农业大学动物医学院，甘肃兰州 730070；3.中国农业科学院兰州畜牧与兽药研究所-农业部兽用药物创制重点实验室，甘肃省新兽药工程重点实验室，甘肃兰州 730050

收稿日期:2011-11-29 修回日期:2012-10-26 出版日期:2012-12-25 发布日期:2012-12-12
通讯作者: 王慧 E-mail:wang_hui_1011@163.com
基金资助:
甘肃省科技支撑项目;甘肃省农业生物技术项目

Optimization of Polysaccharide Extraction from Yeast by Response Surface Methodology

WANG Hui1,2，CHENG Fu-sheng3，LUO Yong-jiang3，DONG Peng-cheng3，ZHANG Xia1,*

1. College of Life Sciences and Technology, Gansu Agricultural University, Lanzhou 730070, China；2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China；3. Key Laboratory of Veterinary Pharmaceutics Discovery, Ministry of Agriculture, Key Laboratory of New Animal Drug Project,Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China

Received:2011-11-29 Revised:2012-10-26 Online:2012-12-25 Published:2012-12-12

摘要/Abstract

摘要：

为提高酵母多糖提取率，对其提取过程进行优化。在单因素试验的基础上，利用中心组合试验设计原理，以高压时间、超声功率和超声时间为试验因素，以多糖提取率为响应值，采用3因素3水平的响应面分析法建立数学模型，获得最佳提取工艺。通过二次回归模型响应面分析得出酵母多糖提取的最佳工艺条件为高压时间35min、超声功率510W、超声时间26min；在此条件下，多糖提取率的预测值为29.82%，验证值为29.84%。证明采用响应面法对酵母多糖提取条件进行优化，方法可行，可用于实际操作与实验预测。

关键词: 酵母多糖, 响应面法, 提取, 优化

Abstract:

Response surface methodology was used to optimize polysaccharide extraction from yeast to enhance polysaccharide yield. Polysaccharide yield was investigated with respect to high pressure treatment time, ultrasonic power and ultrasonic treatment time. A quadratic regression model was established based on a three-variable, three-level Box-Behnken design. The optimum conditions of high pressure treatment time, ultrasonic power and ultrasonic treatment time were found to be 35 min, 510 W and 26 min, respectively. Under these conditions, the predicted value of polysaccharide yield was 29.82%, whereas the actual value was 29.84%. Thus, the optimized extraction procedure is feasible for practical operation and experimental prediction.

Key words: yeast polysaccharide, response surface methodology, extraction, optimization

中图分类号:

王慧1,2，程富胜3，罗永江3，董鹏程3，张霞1,*. 响应面法优化酵母多糖的提取工艺[J]. 食品科学, 2012, 33(24): 92-96.

参考文献

[1]XU W T, ZHANG F F, LUO Y B, et al.Antioxidant activity of a water-soluble polysaccharide purified from pteridium aquilinum[J].Carbohydr Res,2009,344(2):217-222

[2]史怀, 刘波, 苏明星, 等.淡紫拟青霉胞外多糖的分离、纯化及结构分析[J].生物工程学报,2010,26(8):1080-1087

[3]WASSER S P.Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides[J].Appl Microbiol Biotechnol,2002,60:258-274

[4]王允祥, 吕凤霞, 陆兆新.杯伞（Clitocybe sp）培养条件Box-Behnken法优化研究[J].. 中国农业科学,2005,38(1):145-150

[5]FREIMUND S, SAUTER M, KAPPELI O, et al.A new non-degrading isolation process for 1,3-β-d-glucan of high purity from baker's yeast Saccharomyces cerevisiae[J].Carbohydr Polym,2003,54(2):159-171

[6]KOGAN G, KOCHER A.Role of yeast cell wall polysaccharides in pig nutrition and health protection[J].Livestock Sci,2007,109(1-3):161-165

[7]Bohn J A, Bemiller J N.(1→3)-β-D-Glucans as biological response modifiers: a review of structure-functional activity relationships[J].Carbohydr Polym,1995,89(1):3-14

[8]CIPAK L, MIADOKOVA E, DINGOVA H, et al.Comparative DNA protectivity and antimutagenicity studies using DNA-topology and Ames assays[J].Toxicol in Vitro,2001,15(6):677-681

[9]KOGAN G, STASKO A, BAUEROVA K, et al.Antioxidant properties of yeast (1→3)-β-D-glucan studied by electron paramagnetic resonance spectroscopy and its activity in the adjuvant arthritis[J].Carbohydr Polym,2005,61(1):18-28

[10]SLAMENOVA D, LABAJ J, KRIZKOVA L, et al.Protective effects of fungal (1→3)-β-D-glucan derivatives against oxidative DNA lesions in V79hamster lung cells[J].Cancer Lett,2003,198(2):153-160

[11]KHALIKOVA T A, ZHANAEVA S Y, KOROLENKO T A, et al.Regulation of activity of cathepsins B, L, and D in murine lymphosarcoma model at a combined treatment with cyclophosphamide and yeast polysaccharide[J].Cancer Lett,2005,223(1):77-83

[12]KOGAN G, PAJITINKA M, BABINCOVA M, et al.Yeast cell wall polysaccharides as antioxidants and antimutagens: can they fight cancer[J].Neoplasma,2008,55(5):387-393

[13]王元秀, 张桂香, 李峰, 等.酵母多糖的提取及其对雏鸡免疫器官发育的影响[J].食品科学,2011,32(2):225-259

[14]孙海翔, 尹卓容, 马美范.高压均质破碎啤酒酵母细胞壁的研究[J].食品工业科技,2002,23(2):66-67

[15]WASE D A J, PATEL Y R.Effect of cell 35 on disintegration by ultrasonics[J].J Chem Technol Biotechnol. B: biotechnol,1985,35(2):165-173

[16]WILLIAMS D L, PRETUS H A, MCNAMEE R B, et al.Development of a water-soluble, sulfated (1→3)-β-d-glucan biological response modifier derived from Saccharomyces cerevisiae[J].Carbohydr Res,1992,235:247-257

[17]WANG Y J, YAO S J, WU T X.Combination of induced autolysis and sodium hypochlorite oxidation for the production of Saccharomyces cerevisiae (1→3)-β-D-glucan[J].World Journal of Microbiology and Biotechnology,2003,19(9):947-952

[18]AMBATI P, AYYANNA C.Optimizing medium constituents and fermentation conditions for citric acid production from palmyra jaggery using response surface method[J].World J Microbiol Biotechnol,2001,17(4):331-335

[19]SILVA E M, ROGEZ H, LARONDELLE Y.Optimization of extractionof phenolics from Inga edulis leaves using response surface methodology[J].Sep Purif Technol,2007,55(3):381-387

[20]ANNADURAI G, SHEEJA R Y.Use of Box-Behnken design of experiments for the adsorption of verofix red using biopolymer[J].Bioprocess Eng,1998,18(6):463-466

[21]OIJKAAS L P, WILKINSON E C, TRAMPER J, et al.Medium optimization for spore production of coniothyrium minitans using statistically-based experimental designs[J].Biotechnol and Bioeng,1999,64(1):92-100

[22]ISMAIL A, SOULTANI S, GHOUL M.Optimizatin of the enzymatic synthesis of butyl glucoside using response surface methodology[J].Biotehnol Prog,1998,14(6):874-878

[23]LI Q H, FU C L.Application of response surface methodology for extraction optimization of germinant pumpkin seeds protein[J].Food Chem,2005,92(4):701-706

[24]刘烺新, 陈宗道, 王光慈.啤酒酵母胞壁多糖提取工艺研究[J].重庆大学学报,1994,17(6):43-48

[25]WALTHER P, MULLE M.Double-layer coating for field-emission cryo-scanning electron microscopy-Present state and applications[J].Scanning,1997,19(5):343-348

[26]MURALIDHAR R V, CHIRUMAMILA R R, MARCHANT R, et al.A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources[J].Biochem Eng J,2001,9(1):17-23

[27]崔庆新, 刘军海, 黄宝旭, 等.响应面分析法优化党参多糖提取工艺[J].药物分析杂志,2011,31(5):816-820

[28]黄国荣, 朱俊东, 糜漫天, 等.啤酒酵母多糖提取工艺的研究[J].第三军医大学学报,2006,28(6):封2-01

[29]吴小刚, 吴周和, 吴传茂.啤酒酵母多糖提取工艺条件的研究[J].饲料工业,2006,27(9):27-29

响应面法优化酵母多糖的提取工艺

Optimization of Polysaccharide Extraction from Yeast by Response Surface Methodology

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	韩喻，扈莹莹，贺菁蕾，王辉，陈倩，秦立刚. 植物提取物对晚期糖基化终产物抑制机理的研究进展[J]. 食品科学, 2021, 42(9): 233-240.
[2]	王凤，肖楚翔，刘淑珍，王凤舞. 榴莲核黄酮的提取及其对秀丽隐杆线虫氧化和衰老的影响[J]. 食品科学, 2021, 42(9): 123-129.
[3]	张启月，张士凯，郗良卿，杜海云，吴澎. 不同提取方法对樱桃酒渣水溶性膳食纤维结构、理化与功能性质的影响[J]. 食品科学, 2021, 42(7): 98-105.
[4]	郭刚军，胡小静，付镓榕，马尚玄，徐荣，黄克昌，彭志东，贺熙勇，邹建云. 澳洲坚果青皮不同极性溶剂分步提取物功能成分与抗氧化活性及其相关性分析[J]. 食品科学, 2021, 42(7): 74-82.
[5]	王存堂，高增明，姜辰昊，孔保华. 茶叶乙醇提取物对蜡质玉米淀粉回生性质的影响[J]. 食品科学, 2021, 42(6): 53-60.
[6]	刘旺景，李书仪，段嘉钰，丁赫，乔亚杰，刘玉蓉，吕可欣，郭艳丽，敖长金. 沙葱及其提取物对肉羊体脂4-烷基支链脂肪酸沉积与分布的影响[J]. 食品科学, 2021, 42(5): 61-67.
[7]	李伟，叶嘉宜，陈运娇，曹庸. 桉叶多酚提取物体内外抗氧化活性评价[J]. 食品科学, 2021, 42(5): 160-168.
[8]	陈茏，杨俊，马毛毛，吴莎莎，余平，曾哲灵. 产蛋白酶菌株的筛选及以菜籽粕为氮源的产酶条件优化[J]. 食品科学, 2021, 42(4): 115-121.
[9]	张源，贾静娴，郑贺，丁文琪，杨蕊宁. 绿茶提取液制备金纳米粒子及其在VC比色分析中的应用[J]. 食品科学, 2021, 42(4): 107-114.
[10]	马永强，修伟业，黎晨晨，王艺錡，陈俊杰. 星点设计-响应面法优化番茄红素纳米结构脂质载体的制备[J]. 食品科学, 2021, 42(3): 121-127.
[11]	柳萌，郜海燕，房祥军，吴伟杰，陈杭君，刘瑞玲. 不同成熟度杨梅酚酸的超声-微波协同优化提取及其抗氧化性对比[J]. 食品科学, 2021, 42(3): 112-120.
[12]	彭常梅，方锐琳，赖敏，邓泽元，刘小如，李静. 不同提取方法对牡丹籽油品质的影响[J]. 食品科学, 2021, 42(3): 104-111.
[13]	赵磊，潘飞，林文轩，徐美利，鲍玺，陈艳麟，王成涛，连运河. 甜叶菊废渣提取物的主要成分分析及其抗氧化作用[J]. 食品科学, 2021, 42(2): 247-254.
[14]	胡晓龙，王康丽，宋丽丽，侯建光，曹振华，吴丽丽，牛广杰，马歌丽，赵书民，赵东. 浓香型白酒酒醅中总RNA提取方法评价[J]. 食品科学, 2021, 42(2): 74-82.
[15]	杨舒乔，王迪，高彦祥. 非变性II型胶原蛋白研究进展[J]. 食品科学, 2021, 42(17): 343-349.