磁性壳聚糖微球固定化碱性蛋白酶酶学性质

食品科学 ›› 2012, Vol. 33 ›› Issue (21): 249-252.

磁性壳聚糖微球固定化碱性蛋白酶酶学性质

李丹丹1，江连洲2,3,*，李杨2,3，王胜男2，王梅2，李丹2,4

1.江苏省宿迁市宿豫区食品药品监督管理局 2.东北农业大学食品学院 3.国家大豆工程技术研究中心 4.宁德师范学院

收稿日期:2011-08-24 修回日期:2012-09-20 出版日期:2012-11-15 发布日期:2012-11-09
通讯作者: 江连洲 E-mail:jlzname@163.com
基金资助:
黑龙江省攻关项目“大豆蛋白与油脂加工共性关键技术研究与开发”;农业部现代农业产业技术体系建设项目

Enzymatic Properties of Magnetic Chitosan Microspere-Immobilized Alkaline Protease

Received:2011-08-24 Revised:2012-09-20 Online:2012-11-15 Published:2012-11-09

摘要/Abstract

摘要： 以Fe3O4和壳聚糖为原料，以戊二醛为交联剂制备磁性壳聚糖微球，固定化碱性蛋白酶，并对其品貌及结构性质进行观察和分析。结果表明：壳聚糖微球具有良好的球形外貌，大小约为15nm，固定化后粒子大小约为20nm；红外光谱分析证实Fe3O4已被壳聚糖包裹；固定化酶前后粒子晶形完整，均具有良好的磁响应能力和超顺磁性。固定化酶与游离酶相比，最适温度从60℃下降到50℃；最适pH值从10升至11；固定化酶的Km为5.85×10-4mg/mL，游离酶的Km为6.06×10-4mg/mL。

关键词: 壳聚糖, 磁性, 固定, 碱性蛋白酶, 酶学性质

Abstract: Alkaline protease was immobilized onto magnetic chitosan microspheres prepared from Fe3O4 and chitosan by crosslinking with glutaraldehyde and its appearance and structural properties were analyzed. The results showed that the magnetic chitosan microspheres had spherical appearance with a particle size increasing from 15 to 20 nm after immobilization. FT-IR analysis revealed that Fe3O4 was well wrapped up in the magnetic chitosan microspheres. The magnetic chitosan microspheres had complete crystal structure, good magnetic response and strong superparamagnetic behaviors before and after immobilization of alkaline protease. The optimal reaction temperature and pH for the immobilized enzyme were 50 ℃ and 11 compared with 60 ℃ and 10, respectively, for the free enzyme. The Km was 5.85 ×10-4 mg/mL for the immobilized enzyme and 6.06 ×10-4 mg/mL for its free counterpart.

Key words: magnetism, chitosan, immobilization, alkaline protease, enzymatic property

中图分类号:

ts201

李丹丹1，江连洲2,3,*，李?杨2,3，王胜男2，王?梅2，李?丹2,4. 磁性壳聚糖微球固定化碱性蛋白酶酶学性质[J]. 食品科学, 2012, 33(21): 249-252.

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