海南蒲桃果实原花青素的体外抗氧化活性

食品科学 ›› 2012, Vol. 33 ›› Issue (17): 101-105.

海南蒲桃果实原花青素的体外抗氧化活性

张镜，刁树平

嘉应学院生命科学学院

收稿日期:2011-07-22 修回日期:2012-07-31 出版日期:2012-09-15 发布日期:2012-11-09
通讯作者: 张镜 E-mail:zhangcqf@jyu.edu.cn
基金资助:
粤东北林木果实高产活性产物资源的发掘及开发利用

in vitro Antioxidant Activity of Proanthocyanidins in Syzygium cumini Fruits

ZHANG Jing

Received:2011-07-22 Revised:2012-07-31 Online:2012-09-15 Published:2012-11-09
Contact: ZHANG Jing E-mail:zhangcqf@jyu.edu.cn

摘要/Abstract

摘要： 以半制备HPLC纯化的海南蒲桃原花青素为供试样品，研究其体外抗氧化活性。结果表明：海南蒲桃原花青素对DPPH自由基的半抑制剂量IC50 为4.448μg/mL，超氧阴离子自由基(O2－·)的半抑制剂量IC50为5.718μg/mL，羟自由基(·OH)的半抑制剂量IC50 为 60.490μg/mL；海南蒲桃原花青素对亚油酸脂质过氧化半抑制剂量IC50为117.091μg/mL，总抗氧化、还原力明显高于VC；金属离子螯合力高于EDTA。提示海南蒲桃原花青素具有较强的清除自由基与抗氧化活性等体外活性。

关键词: 海南蒲桃, 原花青素, 清除自由基, 抗氧化, 体外

Abstract: The antioxidant activity in vitro of proanthocyanidins purified from S. cumini fruits by semi-preparative HPLC was assessed in the present study. The results showed that the IC50 values of proanthocyanidins from S. cumini fruits obtained in DPPH, superoxide anion and hydroxyl radical assays were 4.448, 5.718 μg/mLand 60.490 μg/mL, respectively. An IC50 of 117.091 μg/mL was obtained in anti-linoleic acid peroxidation assay. The total antioxidant capacity and reducing power were significantly higher than those of vitamin C, and the metal-chelating activity was better than that of EDTA. These findings suggest that proanthocyanidins from S. cumini fruits have strong free radical scavenging and antioxidant activities in vitro.

Key words: S. cumin, proanthocyanidins, radical scavenging activity, antioxidation, in vitro

中图分类号:

TS214.9

张镜，刁树平. 海南蒲桃果实原花青素的体外抗氧化活性[J]. 食品科学, 2012, 33(17): 101-105.

ZHANG Jing. in vitro Antioxidant Activity of Proanthocyanidins in Syzygium cumini Fruits[J]. FOOD SCIENCE, 2012, 33(17): 101-105.

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