硅对干旱胁迫下发菜悬浮细胞生理特性的影响

摘要/Abstract

摘要： 研究硅对干旱胁迫下发菜悬浮细胞（Nostoc flagelliforme suspension cells）渗透物质、保护酶活性和细胞质膜结构功能的影响，探讨硅减轻细胞损伤的机理，为野生发菜培植施硅肥提供理论依据。培养发菜悬浮细胞，在15%(W/V)的聚乙二醇6000胁迫下，研究了不同浓度（0.2、0.4、0.8、1.2、2.0mmol/L）硅酸钠处理对发菜悬浮细胞质膜结构功能、保护酶活性和渗透调节物质的影响。0.4mmol/L硅处理缓解干旱胁迫伤害的效果最好，显著提高了发菜悬浮细胞超氧化物歧化酶（SOD）、过氧化物酶活性(POD)、脯氨酸和可溶性糖含量，显著降低了丙二醛和细胞质膜渗透率。硅缓解发菜悬浮细胞干旱胁迫具有剂量效应，以0.4mmol/L硅处理的效果最好，硅增强发菜悬浮细胞的抗旱性。

关键词: 干旱胁迫, 硅, 发菜悬浮细胞, 保护酶活性, 脯氨酸

Abstract: Effects of drought stress on acticities of protective enzymes and oxidative damage of Nostoc flagelliforme suspension cells were investigated. The active mechanisms of silicon were explored to provide a theoretical basis for alleviating drought stress. The artificial cultivation of Nostoc flagelliforme suspension cells was chosed as the research object,under 15 %(w/v) polyethylene glycol (PEG-6000) simulative drought stress,the effects of Si (as sodium silicate) at the different concentrations (0.2、0.4、0.8、1.2、2.0 mmol/L）on cell enzymatic activities and proline, soluble sugar in Nostoc flagelliforme suspension cells were investigated.The best effect on the alleviation of drought stress damage was observed in the treatment of 0.4 mmol/L Si. At this concentration, activities of protective enzymes (including SOD, POD) and contents of proline and soluble sugar were significantly increased, while malondialdehyde (MDA) content and electrolyte leakage were significantly decreased.The results indicated that dosage effect of Si(as sodium silicate) existed on the alleviation of drought stress in Nostoc flagelliforme suspension cells, and the best alleviating effect on drought stress damage was 0.4 mmol/LSi, which elevated the drought tolerance of Nostoc flagelliforme suspension cells.

Key words: drought stress, Si, Nostoc flagelliforme suspension cells, activities of protective enzymes, proline

中图分类号:

TS201.2

郭金英赵艳丽任国艳易军鹏殷勇. 硅对干旱胁迫下发菜悬浮细胞生理特性的影响[J]. 食品科学, 0, (): 0-0.

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