食品科学 ›› 2013, Vol. 34 ›› Issue (22): 59-63.doi: 10.7506/spkx1002-6630-201322012

• 工艺技术 • 上一篇    下一篇

响应面法优化紫薇花色素提取工艺

晏丽,王立志,麻明友   

  1. 1.吉首大学 林产化工工程湖南省重点实验室,湖南 张家界 427000;2.吉首大学城乡资源与规划学院,湖南 张家界 427000;3.吉首大学实验设备与管理中心,湖南 吉首 416000
  • 收稿日期:2013-01-19 修回日期:2013-10-13 出版日期:2013-11-25 发布日期:2013-12-05
  • 通讯作者: 晏丽 E-mail:27521089@qq.com
  • 基金资助:

    林产化工工程湖南省重点实验室开放基金项目;吉首大学校级科研项目资助

Optimization of Pigment Extraction from Lagerstroemia indica Flowers by Response Surface Methodology

YAN Li,WANG Li-zhi,MA Ming-you   

  1. 1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China;
    2. College of Resource and Planning Sciences, Jishou University, Zhangjiajie 427000, China;
    3. Experimental Equipments Administration Center, Jishou University, Jishou 416000, China
  • Received:2013-01-19 Revised:2013-10-13 Online:2013-11-25 Published:2013-12-05

摘要:

研究紫薇花色素的提取工艺。在单因素试验基础上,根据Box-Behnken试验设计原理采用三因素三水平响应面分析法,优化紫薇花色素超声强化提取工艺。结果表明:紫薇花色素溶液的吸收光谱在λ=546nm处有明显吸收峰,可作为测定紫薇花色素溶液吸光度的特定波长。以体积分数50%乙醇溶液提取花色素的效果较好,超声波辅助提取色素的最佳提取条件为超声功率400W、超声时间22min、液料比45:1(mL/g),在此条件下紫薇花色素吸光度的理论预测值为0.608,实际验证值为0.610,两者误差较小,表明该工艺条件合理可行。

关键词: 紫薇花, 色素, 响应面, 提取

Abstract:

A three-variable, three-level Box-Behnken experimental design combined with response surface analysis was
employed to optimize the conditions for pigment extraction from Lagerstroemia indica flowers. The extracted pigment was
determined by measuring the absorbance at the maximum absorption wavelength of 546 nm. The best solvent for the extraction
of pigment was found to be 50% ethanol. The optimal conditions for ultrasonic-assisted extraction were determined as
400 W ultrasonic power, 22 min irradiation time and a liquid-material ratio of 45:1 (mL/g). The predicted absorbance of pigment
extracts under the optimized conditions was 0.608 and the actual value was 0.610, showing a small error between both.
Thus, the optimized extraction conditions are reliable.

Key words: Lagerstroemia indica flowers, pigment, response surface methodology, extraction

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