食品科学

• 工艺技术 • 上一篇    下一篇

巴参菜多糖提取工艺及抑制HepG2细胞活性

聂志奎,石炜菁,何 慧,周 庆,王 云,张久亮   

  1. 1.华中农业大学食品科学技术学院,湖北 武汉 430070;2.环境食品学教育部重点实验室,湖北 武汉 430070;
    3.华中科技大学同济医学院附属武汉中心医院药学部,湖北 武汉 430014
  • 出版日期:2013-12-25 发布日期:2014-01-03

Extraction and Antitumor Activity of Polysaccharides from Talinum triangulare

NIE Zhi-kui,SHI Wei-jing,HE Hui,ZHOU Qing,WANG Yun,ZHANG Jiu-liang   

  1. 1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
    2. Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, China;3. Department of Pharmacy,
    Wuhan City Central Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China
  • Online:2013-12-25 Published:2014-01-03

摘要:

以巴参菜全草为原料,采用水提醇沉法,通过单因素和L9(34)正交试验考察提取温度、提取时间、料液比对多糖提取率的影响,确定其最佳提取条件;并通过四氮甲唑蓝法和台盼蓝染色法初步评价巴参菜多糖体外抗肿瘤活性。结果表明:巴参菜多糖最佳提取条件为提取温度100℃、提取时间2.0h、料液比1:25(g/mL),提取2次,此条件下多糖提取率为14.99%;巴参菜多糖能显著抑制HepG2细胞增殖、降低其存活率,且呈现出量效关系和时效关系。因此,巴参菜多糖能较好的抑制HepG2细胞活性。

关键词: 巴参菜, 多糖, 提取, 抗肿瘤活性

Abstract:

The extraction of soluble polysaccharides from the whole herb of Talinum triangulare was optimized using onefactor-
at-a-time and L9 (34) orthogonal array design methods. The extraction efficiency of polysaccharides was investigated
as a function of temperature, time and solid-to-solvent ratio. The antitumor activity of the extracted polysaccharides was
evaluated by examining their effects on the proliferation of human cancer HepG2 cells using MTT assay and trypan blue
staining. Results indicated that the optimal conditions for extracting soluble polysaccharides from T. triangulare were
found as follows: extraction temperature of 100 ℃, extraction time of 2.0 h, solid-to-solvent ratio of 1:25 (g/mL) and 2
extraction cycles (repeated twice). The yield of polysaccharides under the optimized conditions was 14.99%. The extracted
polysaccharides exhibited a potent antitumor activity in vitro as indicated by their significant inhibition on the proliferation
of HepG2 cells and reduction of their survival in a concentration- and time-dependent manner.

Key words: Talinum triangulare, polysaccharide, extraction, antitumor activity