玉米籽粒低氧胁迫发芽期间主要生理生化和γ-氨基丁酸含量变化

doi:10.7506/spkx1002-6630-201417021

食品科学

玉米籽粒低氧胁迫发芽期间主要生理生化和γ-氨基丁酸含量变化

尹永祺，吴进贤，刘春泉，李大婧，杨润强，顾振新

1.南京农业大学农业部农畜产品加工与质量控制重点开放实验室，江苏南京 210095；
2.江苏省农业科学院农产品加工研究所，江苏南京 210014

出版日期:2014-09-15 发布日期:2014-09-12

Changes in Physio-biochemical Indexes and γ-Aminobutyric Acid Content during Germination of Maize(Zea mays L.) under Hypoxia Stress

YIN Yong-qi, WU Jin-xian, LIU Chun-quan, LI Da-jing, YANG Run-qiang, GU Zhen-xin

1. Key Laboratory of Agricultural and Animal Products Processing and Quality Control, Ministry of Agriculture,Nanjing Agricultural University, Nanjing 210095, China; 2. Institute of Farm Product Processing,Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Online:2014-09-15 Published:2014-09-12

摘要/Abstract

摘要：

以玉米籽粒为材料研究低氧胁迫下籽粒发芽过程中主要生理生化和γ-氨基丁酸（γ-aminobutyric acid，GABA）含量变化，筛选出最适富集GABA的玉米品种。结果表明：5 个品种玉米籽粒在72 h低氧胁迫发芽期间，芽长增长15.5～26.3 mm，呼吸强度提高1.7～3.2 倍，干物质损失35.6%～40.6%，淀粉消耗15.5%～28.9%，还原糖和游离氨基酸含量分别增加1.7～4.7 倍和7.4～13.3 倍，GABA含量提高9.3～13.2 倍，不同品种玉米籽粒发芽能力和GABA富集量呈显著差异，以京甜紫花糯（ZHN）品种发芽率最高，达到85.5%，且GABA富集量达到0.65 mg/g（以干质量计），ZHN是富集GABA的最适玉米品种。低氧胁迫下发芽玉米中GABA含量与芽长、呼吸强度、游离氨基酸呈极显著正相关（P＜0.01），玉米经低氧胁迫发芽能提升玉米营养品质。

关键词: 玉米籽粒, 发芽, 低氧胁迫, &gamma, -氨基丁酸

Abstract:

The changes in physio-biochemical indexes and γ-aminobutyric acid (GABA) content during germination of
five maize cultivars under hypoxia stress were investigated. Results showed the physiological and biochemical indexes
of five maize cultivars significantly changed during 72 h germination under hypoxia. At the end of germination, the
sprout length increased to 15.5－26.3 mm, the respiratory rate was improved by 1.7－3.2 folds, the dry matter was lost by
35.6%－40.6%, the starch content was consumed by 15.5%－28.9%, and the content of reducing sugar and free amino acids
increased by 1.7－4.7 and 7.4－13.3 folds, respectively. GABA content in germinated maize increased by 9.3－13.2 folds
when compared with the original maize. Furthermore, significant differences in GABA content and germination rate among
different maize cultivars during germination were observed. Germination rate of cultivar ZHN was the highest (85.5%) with
a maximum GABA content of 0.65 mg/g md. Hence, ZHN could be considered as a good source for GABA accumulation.
GABA content in maize during germination under hypoxia revealed a significantly (P ＜ 0.01) positive correlation with
sprout length, respiration rate and free amino acid content, respectively. The nutritional quality of maize could be improved
through germination under hypoxia stress.

Key words: maize, germination, hypoxia stress, γ-aminobutyric acid

中图分类号:

TS210.1

尹永祺，吴进贤，刘春泉，李大婧，杨润强，顾振新. 玉米籽粒低氧胁迫发芽期间主要生理生化和γ-氨基丁酸含量变化[J]. 食品科学, doi: 10.7506/spkx1002-6630-201417021.

YIN Yong-qi, WU Jin-xian, LIU Chun-quan, LI Da-jing, YANG Run-qiang, GU Zhen-xin. Changes in Physio-biochemical Indexes and γ-Aminobutyric Acid Content during Germination of Maize(Zea mays L.) under Hypoxia Stress[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201417021.

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玉米籽粒低氧胁迫发芽期间主要生理生化和γ-氨基丁酸含量变化

Changes in Physio-biochemical Indexes and γ-Aminobutyric Acid Content during Germination of Maize(Zea mays L.) under Hypoxia Stress

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