小白菜硝酸盐含量与NO3－/NH4+及氮代谢关键酶的相关性

doi:10.7506/spkx1002-6630-201523014

摘要/Abstract

摘要：

采用液体培养实验研究了不同NO3－与NH4＋浓度比（分别为50∶50、75∶25和100∶0）对10 个品种小白菜（Brassica chinensis L.）的硝酸盐含量、光合系统参数及氮代谢关键酶活性的影响。结果表明，随着硝铵比的增加，小白菜总干质量逐渐增加（除品种天津小白菜和大头清江白菜的总干质量随硝铵比的增加先降低后升高，品种香港春秀甜白菜（236）和正旺达88的总干质量先升高后降低外），与c（NO3－）∶c（NH4＋）为50∶50比较，增幅分别为12.66%～76.88%（c（NO3－）∶c（NH4＋）为75∶25）和17.98%～95.07%（c（NO3－）∶c（NH4＋）为100∶0）；小白菜叶和根中的硝酸盐含量增加，而叶柄硝酸盐含量则表现出先增加后下降的趋势，小白菜硝酸盐的含量叶柄（3 882.62～5 448.81 mg/kg）＞叶（2 004.86～4 146.50 mg/kg）＞根（505.39～2 188.68 mg/kg）。小白菜叶片中硝酸盐含量与硝酸还原酶活性成显著负相关，而与亚硝酸还原酶和谷氨酰胺合成酶活性成显著正相关。小白菜叶片中硝酸盐的含量随着净光合速率（Pn）和胞间二氧化碳浓度（Ci）的增加而下降，随蒸腾速率（Tr）的增强而增加。

关键词: 小白菜品种, 硝酸盐, NO3－/NH4＋, 氮代谢关键酶, 相关性

Abstract:

Solution culture experiments were carried to explore the relationships between nitrate contents, parameters
relevant to photosynthesis and key enzymes involved in nitrogen metabolism in ten bok choy varieties (Brassica chinensis L.)
by setting NO3-/NH4+ ratio as 50:50, 75:25 and 100:0. The results showed that six out of ten bok choy varieties investigated
indicated an increase in total dry weight respectively by 12.66%-76.88% and 17.98%-95.07% at NO3-/NH4+ ratio of 75:25
and 100:0 compared with that at NO3-/NH4+ ratio of 50:50, though total dry weight of the Tianjin and Datouqingjiang
varieties decreased firstly and then increased whereas the Xianggang Chunxiu Tianbaicai 236 and Zhengwangda 88 varieties
exhibited an initial increase followed by a decrease with increasing NO3-/NH4+ ratio. Nitrate contents of leaves and roots
of bok choy rose, while those of petiole increased initially and then decreased. Moreover, the decreasing order of nitrate
contents was petiole (3 882.62–5 448.81 mg/kg) > leaf (2 004.86–4 146.50 mg/kg) > root (505.39–2 188.68 mg/kg).
Increasing NO3-/NH4+ ratio could result in an increase in nitrate contents of leaves and roots of most bok choy varieties and
an initial increase and then decrease in petiole. Significantly negative correlation was observed between nitrate reductase (NR)
and nitrate content in leaves of bok choy, while there was significantly positive correlation between nitrite reductase (NiR)
and glutamine synthetase (GS). Nitrate content of bok choy leaves decreased with an increase in net photosynthetic rate (Pn)
and intercellular CO2 concentration (Ci), while enhancing along with an increase in transpiration (Tr).

Key words: bok choy varieties, nitrate, NO3-/NH4+ ratio, key enzymes involved in nitrogen metabolism, correlation

中图分类号:

迟荪琳，杨芸，徐卫红，陈序根，陈永勤，谢文文，熊仕娟，王正银，谢德体. 小白菜硝酸盐含量与NO3－/NH4+及氮代谢关键酶的相关性[J]. 食品科学, doi: 10.7506/spkx1002-6630-201523014.

CHI Sunlin, YANG Yun, XU Weihong, CHEN Xugen, CHEN Yongqin, XIE Wenwen, XIONG Shijuan, WANG Zhengyin, XIE Deti. Relationships between Nitrate Contents and NO3-/NH4+ Ratio or Key Enzymes of Nitrogen Metabolism in Bok Choy (Brassica rapa var. chinensis)[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201523014.

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小白菜硝酸盐含量与NO3－/NH4+及氮代谢关键酶的相关性

Relationships between Nitrate Contents and NO3-/NH4+ Ratio or Key Enzymes of Nitrogen Metabolism in Bok Choy (Brassica rapa var. chinensis)

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