家蚕1-脱氧野尻霉素含量与体内糖苷酶活性的相关性

doi:10.7506/spkx1002-6630-201615026

食品科学

家蚕1-脱氧野尻霉素含量与体内糖苷酶活性的相关性

陈红霞1,2，刘月琴2，潘刚3，赵明2，欧阳臻1,2,*

1.江苏大学食品与生物工程学院，江苏镇江 212013；2.江苏大学药学院，江苏镇江 212013；3.中国农业科学院蚕业研究所，江苏镇江 212018

出版日期:2016-08-15 发布日期:2016-08-30

Correlation between 1-Deoxynojirimycin Content and Glucosidase Activities in Silkworm (Bombyx mori L.)

CHEN Hongxia1,2, LIU Yueqin2, PAN Gang3, ZHAO Ming2, OUYANG Zhen1,2,*

1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; 2. School of Pharmacy, Jiangsu University, Zhenjiang 212013, China; 3. The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China

Online:2016-08-15 Published:2016-08-30

摘要/Abstract

摘要：

为研究家蚕富集1-脱氧野尻霉素（1-deoxynojirimycin，DNJ）的机制，本实验分析了不同品种家蚕体内DNJ含量并研究其与蚕体内糖苷酶的关系。选择了25 个不同的家蚕品种，用相同条件的桑叶饲喂不同品种的家蚕，采用柱前衍生高效液相色谱法分析不同品种蚕体DNJ含量并研究其与体内蔗糖酶、乳糖酶及淀粉酶3 种糖苷酶活性之间的相关性。结果表明：不同品种家蚕体内DNJ含量差异显著，DNJ含量最高种是最低种的7 倍，蚕体DNJ含量与体内的蔗糖酶、乳糖酶等二糖酶活力成显著负相关，与淀粉酶的活力相关性相对较弱。

关键词: 家蚕, 1-脱氧野尻霉素, 糖苷酶, 相关性

Abstract:

In order to reveal the mechanism of 1-deoxynojirimycin (DNJ) enrichment in silkworm (Bombyx mori L.), we
studied and analyzed the correlation between DNJ content and glucosidase activities in silkworm. Towards this goal, 25
strains of silkworms were selected and raised with the same mulberry leaves. Pre-column derivatization high performance
liquid chromatography (HPLC) was applied to determine the DNJ content of different strains of silkworms, and the activities
of 3 major glucosidases (sucrase, lactase and amylase) were measured, respectively, for the analysis of correlation between
DNJ content and glucosidase activities in silkworms. Our results indicated that different strains of silkworms had significant
differences in DNJ content, and the highest DNJ content (which was found in the strain 2042, 0.56%) was 7 times higher
than the lowest one (in ‘DongTing × BiBo’, 0.08%). DNJ content had significantly negative correlations with the activity of
either of two disaccharidases, sucrase and lactase, and had a relatively weaker correlation with amylase activity.

Key words: Bombyx mori L., 1-deoxynojirimycin, glucosidase, correlation

中图分类号:

R932

陈红霞，刘月琴，潘刚，赵明，欧阳臻. 家蚕1-脱氧野尻霉素含量与体内糖苷酶活性的相关性[J]. 食品科学, doi: 10.7506/spkx1002-6630-201615026.

CHEN Hongxia, LIU Yueqin, PAN Gang, ZHAO Ming, OUYANG Zhen. Correlation between 1-Deoxynojirimycin Content and Glucosidase Activities in Silkworm (Bombyx mori L.)[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201615026.

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家蚕1-脱氧野尻霉素含量与体内糖苷酶活性的相关性

Correlation between 1-Deoxynojirimycin Content and Glucosidase Activities in Silkworm (Bombyx mori L.)

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