乌鲁木齐牛羊肉源沙门氏菌对喹诺酮类药物的耐药状况及相关基因分析

doi:10.7506/spkx1002-6630-201704018

食品科学 ›› 2017, Vol. 38 ›› Issue (4): 107-112.doi: 10.7506/spkx1002-6630-201704018

乌鲁木齐牛羊肉源沙门氏菌对喹诺酮类药物的耐药状况及相关基因分析

葛琨，武运，杨保伟，吴浩天，王威，张亚南，田歌，马文瑞

1.新疆农业大学食品科学与药学学院，新疆乌鲁木齐 830052； 2.西北农林科技大学食品科学与工程学院，陕西杨凌 712100

出版日期:2017-02-25 发布日期:2017-02-28
基金资助:
新疆维吾尔自治区“十三五”重大专项（2016A01001-5）

Quinolone Resistance Characteristics and Related Gene Analysis of Salmonella in Beef and Mutton Retailed in ürümqi

GE Kun, WU Yun, YANG Baowei, WU Haotian, WANG Wei, ZHANG Yanan, TIAN Ge, MA Wenrui

1. College of Food Science and Pharmaceutical Science, Xinjiang Agricultural University, ürümqi 830052, China; 2. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China

Online:2017-02-25 Published:2017-02-28

摘要/Abstract

摘要： 目的：研究牛羊肉源沙门氏菌对15 种抗生素的药敏性以及对喹诺酮类药物的相关耐药基因，更好地了解沙门氏菌耐药性的产生和传播途径，为预防与控制沙门氏菌疾病提供基础信息。方法：用琼脂稀释法测定沙门氏菌的药敏性，用聚合酶链式反应和基因序列测定法确定耐药沙门氏菌中与喹诺酮类抗生素耐药相关的喹诺酮类抗性决定区基因突变及质粒携带的耐药基因。结果：30 株沙门氏菌中对甲氧苄啶、氯霉素、萘啶酮酸、四环素、磺胺异甲二唑、链霉素、甲氧嘧啶/磺胺异恶唑、阿莫西林、氨苄西林的耐药率分别为100%、86.7%、66.7%、60.0%、50.0%、33.3%、26.7%、6.7%、6.7%，对环丙沙星、头孢曲松、庆大霉素、卡那霉素、头孢西丁、阿米卡星均表现为敏感；qnrB、qnrA、qnrS、aac(6′)-Ib-cr基因的检出率分别为5.0%、45.0%、0%、5.0%；30 株沙门氏菌发生gyrA基因突变的菌株数为14 株，主要突变类型是Ser83Phe，发生parC基因突变的菌株数为25 株，主要突变类型是Thr57Ser。结论：乌鲁木齐牛羊肉源沙门氏菌的耐药情况较为严重，对喹诺酮类药物的耐药状况应当予以关注，其耐药突变基因及质粒携带的耐药基因在一定程度上是导致沙门氏菌耐药的重要原因。

关键词: 沙门氏菌, 药敏性, 喹诺酮, 耐药基因

Abstract: Objective: To determine the antibacterial susceptibility to 15 antibiotics and quinolone resistance genes of 30 Salmonella isolates from beef and mutton ürümqi, Xinjiang for the purpose of better understanding the development and transmission pathways of antibiotic resistance in Salmonella and hence providing basic information for preventing and controlling Salmonella-related diseases. Methods: The drug sensitivity of Salmonella isolates was evaluated by the agar dilution method. In addition, polymerase chain reaction and gene sequencing were used to detect the quinolone resistancedetermining regions mutations and the plasmid-mediated quinolone resistance genes. Results: The drug resistance rates of 30 Salmonella isolates to trimethoprim, chloramphenicol nalidixic acid, tetracycline, sulfisoxazole, streptomycin, trimethoprim/ sulfamethoxazole, amoxicillin/clavulanic, ampicillin were 100%, 86.7%, 66.7%, 60.0%, 50.0%, 33.3%, 26.7%, 6.7% and 6.7%, respectively. All the Salmonella isolates were sensitive to ciprofloxacin, ceftriaxone, gentamicin, kanamycin, cefoxitin and amikacin. The number of Salmonella carrying gyrA mutations was 14, and the main type of mutation was Ser83Phe; the number of Salmonella having parC mutations was 25, and the main type of mutation was Thr57Ser. Conclusions: The Salmonella isolates from beef and mutton retailed in ürümqi are resistant to multiple antibiotics. Therefore, we should be seriously concerned about this phenomenon. The antibiotic resistance mutations and the plasmid-mediated quinolone resistance genes may be an important cause of the antimicrobial resistance of Salmonella isolates from beef and mutton in some extend.

Key words: Salmonella, antibacterial susceptibility, quinolone, antibiotic resistance gene

中图分类号:

TS201.3

葛琨，武运，杨保伟，吴浩天，王威，张亚南，田歌，马文瑞. 乌鲁木齐牛羊肉源沙门氏菌对喹诺酮类药物的耐药状况及相关基因分析[J]. 食品科学, 2017, 38(4): 107-112.

GE Kun, WU Yun, YANG Baowei, WU Haotian, WANG Wei, ZHANG Yanan, TIAN Ge, MA Wenrui. Quinolone Resistance Characteristics and Related Gene Analysis of Salmonella in Beef and Mutton Retailed in ürümqi[J]. FOOD SCIENCE, 2017, 38(4): 107-112.

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乌鲁木齐牛羊肉源沙门氏菌对喹诺酮类药物的耐药状况及相关基因分析

Quinolone Resistance Characteristics and Related Gene Analysis of Salmonella in Beef and Mutton Retailed in ürümqi

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