食品科学 ›› 2011, Vol. 32 ›› Issue (9): 18-21.doi: 10.7506/spkx1002-6630-201109004

• 基础研究 • 上一篇    下一篇

鲜鸡蛋中溶菌酶的活性分布

仝其根,唐巍巍,宋美娜   

  1. 北京农学院食品科学学院
  • 出版日期:2011-05-15 发布日期:2011-04-11
  • 基金资助:
    2008 年北京市教委科研项目(M200810020003)

Distribution of Lysozyme Activity in Fresh Eggs

TONG Qi-gen,TANG Wei-wei,SONG Mei-na   

  1. College of Food Science, Beijing University of Agriculture, Beijing 102206, China
  • Online:2011-05-15 Published:2011-04-11

摘要: 为了研究鸡蛋不同部位溶菌酶的活性,在不破坏蛋体内部结构的情况下,利用注射器和照蛋器抽取蛋内样品(系带与蛋黄膜除外),对鲜鸡蛋各个部分的溶菌酶活性进行分析研究。结果表明:壳外膜及蛋黄中不含溶菌酶,蛋黄膜所含溶菌酶活力最高为9764.290U/mL,其次系带为4172.86U/mL,系带中溶菌酶活力约为其他部位溶菌酶活力的11倍以上;蛋膜黏附蛋白、外稀蛋白、中浓蛋白和内稀蛋白这4个部位所含溶菌酶活力分别为378.714、 330.238、354.929、245.381U/mL,壳外膜和蛋黄不含有溶菌酶。蛋清在蛋内的位置不同,其中溶菌酶活力也存在差异,尖中部及钝端的溶菌酶活性高,而尖端及蛋的中部溶菌酶活性低。

关键词: 鸡蛋, 溶菌酶活性, 分布

Abstract: In order to explore lysozyme activity in different parts of eggs, egg internal content except yolk membrane and chalaza was harvested using syringe and light shining device without damage. Lyozyme activities in different parts of eggs were determined. The results indicated that outer shell albuginea and yolk did not contain lysozyme. Lysozyme activity in yolk membrane was the highest, which was up to 9764.290 U/mL, and then followed by 4172.86 U/mL in chalazae. Loysozyme activities in four parts such as eggshell membrane adhesion albumen, outer thin albumen, inner thick albumen and inner thin albumen were 378.714, 330.238, 354.929 U/mL and 245.381 U/mL, respectively. No lysozyme was observed in outer shell membrane and yolk. Moreover, different lysozyme activities in different locations of eggs were observed. The sharp-middle and blunt end revealed the highest lysozyme activity. The tip end and middle parts of eggs revealed lower lysozyme activity.

Key words: egg, lysozyme activity, distribution

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