虫草与富硒虫草多糖体外抗氧化活性

doi:10.7506/spkx1002-6630-201109017

食品科学 ›› 2011, Vol. 32 ›› Issue (9): 76-78.doi: 10.7506/spkx1002-6630-201109017

虫草与富硒虫草多糖体外抗氧化活性

武忠伟，许桂芳，曹蓬勃，耿杰

1.河南科技学院生物工程系 2.辽宁大学生命科学院

出版日期:2011-05-15 发布日期:2011-04-11
基金资助:
河南省新乡市科技发展项目(09G045)；河南科技学院重点科研基金资助项目(5048)

Antioxidant Activity of Intracellular and Extracellular Polysaccharides from Regular and Se-rich Cordyceps sinensis in vitro

WU Zhong-wei1，XU Gui-fang1，CAO Peng-bo2，GENG Jie1

1. Department of Bioengineering, Henan Institute of Science and Technology, Xinxiang 453003, China； 2.School of Life Science, Liaoning University, Shenyang 110036, China

Online:2011-05-15 Published:2011-04-11

摘要/Abstract

摘要： 采用Fenton法和邻苯三酚自氧化法测定虫草和富硒虫草多糖对 ·OH、O2－ ·和H2O2的清除率。结果表明：虫草多糖对O2－ ·和H2O2的清除能力依次为富硒虫草胞外多糖＞富硒虫草胞内多糖＞虫草胞外多糖＞虫草胞内多糖，而对 ·OH的清除能力依次为：富硒虫草胞外多糖＞虫草胞外多糖＞富硒虫草胞内多糖＞虫草胞内多糖；当100mg/L多糖溶液添加量分别高于80、40、90μL时，各组多糖对 ·OH、O2－ ·和H2O2 3种自由基的抑制率均可高于50%。

关键词: 虫草多糖, 富硒, 体外, 自由基, 抗氧化

Abstract: The scavenging effects of polysaccharides from the mycelia (intracellular) and culture supernatant (extracellular) of Cordyceps sinensis after 6 days of cultivation were determined against hydroxyl free radicals generated in a Fenton reaction system, superoxide anion free radicals (by pyrogallol auto-oxidation method) and hydrogen peroxide. The extracellular polysaccharides from Se-rich Cordyceps sinensis had the strongest ability to scavenge superoxide anion free radicals and hydrogen peroxide, followed by the intracellular polysaccharides from Se-rich Cordyceps sinensis as well as the extracellular and the intracellular polysaccharides from common Cordyceps sinensis. By the scavenging effect against hydroxyl free radicals, the four polysaccharides could be ranked in the following order: the extracellular polysaccharides from Se-rich Cordyceps sinensis, the extracellular polysaccharides from common Cordyceps sinensis, the intracellular polysaccharides from Se-rich Cordyceps sinensis and the intracellular polysaccharides from common Cordyceps sinensis. Adding each of the four polysaccharide solutions (100 mg/L) at a level of no less than 80, 40μL and 90μL had a scavenging rate of over 50% against hydroxyl and superoxide anion free radicals as well as hydrogen peroxide, respectively.

Key words: polysaccharides from Cordyceps sinensis, Se-rich Cordyceps sinensis, radicals, antioxidation in vitro

中图分类号:

TQ929.2

武忠伟，许桂芳，曹蓬勃，耿杰. 虫草与富硒虫草多糖体外抗氧化活性[J]. 食品科学, 2011, 32(9): 76-78.

WU Zhong-wei，XU Gui-fang，CAO Peng-bo，GENG Jie. Antioxidant Activity of Intracellular and Extracellular Polysaccharides from Regular and Se-rich Cordyceps sinensis in vitro[J]. FOOD SCIENCE, 2011, 32(9): 76-78.

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虫草与富硒虫草多糖体外抗氧化活性

Antioxidant Activity of Intracellular and Extracellular Polysaccharides from Regular and Se-rich Cordyceps sinensis in vitro

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