食品科学 ›› 2011, Vol. 32 ›› Issue (10): 21-24.doi: 10.7506/spkx1002-6630-201110005

• 工艺技术 • 上一篇    下一篇

山葡萄籽中原花青素提取工艺优化

王新伟,赵 滢,沈育杰*   

  1. 中国农业科学院特产研究所
  • 出版日期:2011-05-25 发布日期:2011-04-08
  • 基金资助:
    农业部作物种质资源保护项目(NB2010-2130135-36);国家现代农业产业技术体系建设专项(nycytx-30-01)

Optimization of Extraction Process for Proanthicyanidins from Amur Grape Seeds

WANG Xin-wei,ZHAO Ying,SHEN Yu-jie*   

  1. Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jinlin 132109, China
  • Online:2011-05-25 Published:2011-04-08

摘要: 目的:通过单因素与正交试验研究不同的提取温度、料液比、时间、溶剂体积分数等因素对原花青素提取率的影响,确定最佳提取工艺参数。方法:以山葡萄籽为原料,乙醇作为提取剂,通过香草醛-HPLC法测定提取物中原花青素的提取率,香草醛法测定黄烷-3-醇单体与原花青素的总含量,HPLC法测定黄烷-3-醇单体的含量,二者之差为原花青素总含量。结果:最佳工艺参数为乙醇体积分数70%、提取时间120min、提取温度60℃、料液比1:5(g/mL)。结论:在此工艺参数下,原花青素提取率为3.12%。

关键词: 山葡萄籽, 原花青素, 提取, 香草醛, HPLC

Abstract: Objective: To investigate the effects of temperature, time and ethanol concentration and material/liquid ratio on proanthicyanidins extraction from Amur grape seeds through single-factor and orthogonal tests and to explore the optimal extraction process. Methods: Ethanol was used for the extraction of proanthicyanidins. The total content of flavan-3-ol monomers and proanthicyanidins (both of them can be simultaneously extracted by ethanol) was determined by vanillin method, and the method used to determine the content of flavan-3-ol monomers was HPLC. Thus, the content of proanthicyanidins could be calculated as the difference between the two determined values. Results: The optimal extraction process conditions were extraction temperature of 60 ℃, ethanol concentration of 70%, extraction time of 120 min and material/liquid ratio of 1:5 (g/mL). Conclusion: Under the optimal extraction conditions, the extraction rate of proanthicyanidins from Amur grape seeds was up to 3.12%.

Key words: Amur grape seeds, proanthocyanidins, extraction, vanillin, HPLC

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