食品科学 ›› 2011, Vol. 32 ›› Issue (17): 254-257.doi: 10.7506/spkx1002-6630-201117053

• 生物工程 • 上一篇    下一篇

瘤胃细菌生物合成共轭亚油酸的累积特性

刘晓华,李海星,陈 燕,曹郁生*   

  1. 南昌大学中德联合研究院,食品科学与技术国家重点实验室
  • 发布日期:2011-08-30
  • 基金资助:
    江西省教育厅科研项目(赣教技字[2007]38 号)

Accumulation Properties of Conjuagted Linoleic Acid Isomers Biosynthesized by Ruminal Bacteria

LIU Xiao-hua,LI Hai-xing,CHEN Yan,CAO Yu-sheng*   

  1. (State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China)
  • Published:2011-08-30

摘要: 为了解瘤胃细菌生物合成共轭亚油酸(CLA)的特性,通过毛细管电泳(CE)分析,发现瘤胃细菌生物合成CLA的主要异构体有c9,t11-CLA、t10,c12-CLA和t9,t11-CLA 3种。在厌氧和有氧条件下,瘤胃细菌均能合成CLA,且氧气有利于CLA的累积,随反应时间的延长,CLA的量呈现先增加后减少的变化趋势。结果表明瘤胃细菌参与了反刍动物体内CLA异构体的生物合成与代谢,瘤胃细菌生物合成CLA异构体的特异性还有待更深入的研究。

关键词: 瘤胃细菌, 生物转化, 共轭亚油酸, 异构体, 毛细管电泳

Abstract: In order to understand accumulation properties of conjugated linoleic acid (CLA) biosynthesized by ruminal bacteria, capillary electrophoresis (ce)was used to analyze CLA isomers. Three main isomers including c9, t11-CLA, t10, c12-CLA and t9, t11-CLA were synthesized by ruminal bacteria under both anaerobic and aerobic conditions. Aerobic environment was more beneficial for the accumulation of CLA than anaerobic environment. The yield of CLA exhibited a trend of initial increase and final decrease. Therefore, ruminal bacteria play key roles in the biosynthesis and metabolism of CLA in rumen.

Key words: ruminal bacteria, biosynthesis, conjugated linoleic acid, isomer, capillary electrophoresis

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