食品科学 ›› 2018, Vol. 39 ›› Issue (10): 145-150.doi: 10.7506/spkx1002-6630-201810023

• 生物工程 • 上一篇    下一篇

Cu2+对多粘类芽孢杆菌增殖及其转化人参皂苷的影响

肖茹雪,臧埔*,郜玉钢*,赵岩,祝洪艳,何忠梅,杨鹤,刘双利,张连学   

  1. (吉林农业大学中药材学院,吉林?长春 130118)
  • 出版日期:2018-05-25 发布日期:2018-05-15
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFC0500300); 吉林省科技厅科技发展计划项目(20160307005YY;20170204017YY);公益性行业(农业)科研专项(201303111)

Effects of Cu2+ on Paenibacillus polymyxa Proliferation and Conversion of Ginsenosides

XIAO Ruxue, ZANG Pu*, GAO Yugang*, ZHAO Yan, ZHU Hongyan, HE Zhongmei, YANG He, LIU Shuangli, ZHANG Lianxue   

  1. (College of Traditional Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China)
  • Online:2018-05-25 Published:2018-05-15

摘要: 目的:研究Cu2+对多粘类芽孢杆菌增殖及转化人参皂苷的影响。方法:采用酶标仪和高效液相色谱仪分别测定加入Cu2+后多粘类芽孢杆菌的增殖及转化人参皂苷情况。结果:Cu2+促进多粘类芽孢杆菌增殖适宜质量浓度为10~50?mg/L,Cu2+质量浓度为30?mg/L时促进多粘类芽孢杆菌增殖效果最好,增殖时间最短,与对照组相比提前1?d,生物量显著增加;转化体系中Cu2+质量浓度为10~50?mg/L时,促进多粘类芽孢杆菌转化人参皂苷,Cu2+质量浓度为30?mg/L时,多粘类芽孢杆菌转化人参皂苷能力最强;但Cu2+质量浓度超过100?mg/L时反而抑制多粘类芽孢杆菌转化人参皂苷,并且随Cu2+质量浓度增大而抑制作用增强。结论:Cu2+质量浓度为30?mg/L时,可促进多粘类芽孢杆菌增殖及其转化人参皂苷。

关键词: 多粘类芽孢杆菌, Cu2+, 生长曲线, 转化皂苷, 增殖

Abstract: Objective: This study aimed to examine the effect of Cu2+ on the proliferation of Paenibacillus polymyxa and the conversion of ginsenosides. Methods: A microplate reader and HPLC were used for this study. Results: Cu2+ at 10–50 mg/L improved P. polymyxa proliferation and the best effect was observed at 30 mg/L, where it entered the logarithmic growth phase earlier 1 d and the biomass was significantly increased as compared with the control group. The presence of Cu2+ at 10–50 mg/L in the culture medium improved?the conversion of ginsenosides by P. polymyxa and its effect was strongest at 30 mg/L. However, when the concentration of Cu2+ exceeded 100 mg/L, the conversion of ginsenosides was inhibited, and this inhibition was increased with increasing Cu2+ concentration. Conclusion: A Cu2+ concentration of 30 mg/L improved P. polymyxa proliferation and the conversion of ginsenosides.

Key words: Paenibacillus polymyxa, Cu2+, growth curve, conversion of ginsenosides, proliferation

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