食品科学 ›› 2018, Vol. 39 ›› Issue (10): 173-178.doi: 10.7506/spkx1002-6630-201810027

• 生物工程 • 上一篇    下一篇

短乳杆菌天然质粒分类

孙大庆1,2,李洪飞1,杨健2,宋大巍1,3   

  1. (1.黑龙江八一农垦大学?国家杂粮工程技术研究中心,黑龙江 大庆 163319;2.黑龙江八一农垦大学?牡丹江食品与生物技术创新研究院,黑龙江?牡丹江 157000;3.黑龙江八一农垦大学食品学院,黑龙江?大庆 163319)
  • 出版日期:2018-05-25 发布日期:2018-05-15
  • 基金资助:
    黑龙江省青年科学基金项目(QC2014C020);牡丹江市科技计划项目(Z2016n0015); 黑龙江八一农垦大学“校内培育课题资助计划”项目(XZR2016-15)

Classification of Natural Plasmids in Lactobacillus brevis

SUN Daqing1,2, LI Hongfei1, YANG Jian2, SONG Dawei1,3   

  1. (1. National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. Mudanjiang Food and Biotechnology Innovation Institute, Heilongjiang Bayi Agricultural University, Mudanjiang 157000, China; 3. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China)
  • Online:2018-05-25 Published:2018-05-15

摘要: 为建立短乳杆菌天然质粒分类方法,通过质粒复制起始蛋白(replication initiation protein,Rep)进化树和基因组共线性分析,将短乳杆菌51 个天然质粒可以准确、有效地划分为6?个家族类型和11?个亚家族类型,并在家族4质粒中发现了一种新的复制子类型。研究结果表明,质粒Rep进化树和基因组共线性分析都是短乳杆菌天然质粒有效的分类方法,分类结果为今后短乳杆菌天然质粒科学分类和理性应用提供了理论研究依据。

关键词: 短乳杆菌, 质粒, 复制起始蛋白, 基因组共线性, 复制子

Abstract: This study aimed to establish a method for the classification of natural plasmids from Lactobacillus brevis. A total of 51 natural plasmids of L. brevis were accurately and efficiently divided into 6 family types and 11 subfamily types through the replication initiation protein (Rep) phylogenetic tree and genome colinearity analysis. A new type of replicon was found in the plasmids of family 4. The results showed that both the Rep phylogenetic tree and genome colinearity analysis were effective methods for the classification of natural plasmids in L. brevis. The classification results will provide a theoretical foundation for the scientific classification and reasonable application of L. brevis natural plasmids in the future.

Key words: Lactobacillus brevis, plasmid, replication initiation protein, genome colinearity, replicon

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