食品科学 ›› 2018, Vol. 39 ›› Issue (15): 128-131.doi: 10.7506/spkx1002-6630-201815019

• 食品工程 • 上一篇    下一篇

高压微射流处理对原花青素平均聚合度和抗氧化性能的影响

铁珊珊,刘梦培,纵 伟*,张丽华,赵光远   

  1. 郑州轻工业学院食品与生物工程学院,河南 郑州 450002
  • 出版日期:2018-08-15 发布日期:2018-08-15
  • 基金资助:
    河南省科技创新杰出人才计划项目(174200510002);河南省高校科技创新项目(16IRTSHN010)

Effect of Dynamic High-Pressure Microfluidization on the Degree of Polymerization and Antioxidant Capacity of Proanthocyanidins

TIE Shanshan, LIU Mengpei, ZONG Wei*, ZHANG Lihua, ZHAO Guangyuan   

  1. School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China
  • Online:2018-08-15 Published:2018-08-15

摘要: 为提高原花青素的生物利用度,本研究采用高压微射流技术处理原花青素溶液,探究其对原花青素平均 聚合度及其抗氧化能力的影响。将原花青素在不同处理压力(100、140、180、220 MPa和260 MPa)下分别进行 1、2、3、4 次循环处理,结果表明:随着处理压力的升高,原花青素的平均聚合度基本不存在显著差异,220 MPa 下平均聚合度相对较低;处理次数为4条件下的平均聚合度相对较小。固定处理次数为4,选择不同处理压力的样 品进行抗氧化能力分析,抗氧化能力(包括铁还原力和清除1,1-二苯基-2-三硝基苯肼自由基能力)由大到小为: 220 MPa>180 MPa>140 MPa>100 MPa>260 MPa>对照组>VC;半抑制浓度的变化趋势与抗氧化能力大小顺序 相反,说明处理压力为220 MPa下的原花青素抗氧化能力最强。

关键词: 高压微射流, 原花青素, 平均聚合度, 铁离子还原力, 1, 1-二苯基-2-三硝基苯肼自由基清除能力, 半抑制浓度

Abstract: In order to improve the bioavailability of proanthocyanidins, the effect of dynamic high-pressure microfluidization (DHPM) on the average degree of polymerization (DP) and antioxidant capacity of proanthocyanidins was studied. For this purpose, proanthocyanidins were subjected to different DHPM cycles (1, 2, 3 and 4) at different pressure levels (100, 140, 180, 220 and 260 MPa). Results showed that the average DP value of proanthocyanidins was not significantly changed with increasing pressure level, being lower when the processing pressure was 220 MPa. After 4 DHPM cycles, the average DP values were relatively smaller, and the antioxidant capacity (including ferrous ion chelating activity and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity) of proanthocyanidins treated at different pressure levels followed the decreasing order of 220 MPa > 180 MPa > 140 MPa > 100 MPa > 260 MPa > control group > VC. The opposite was observed for the half maximal inhibitory concentration (IC50) values, indicating that the antioxidant capacity of proanthocyanidins treated at 220 MPa was stronger than that of other samples.

Key words: dynamic high-pressure microfluidization, proanthocyanidins, degree of polymerization, ferrous ion reducing activity, 1,1-diphenyl-2-picrylhydrazyl radical scavenging capacity, half maximal inhibitory concentration

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