食品科学 ›› 2012, Vol. 33 ›› Issue (10): 24-28.doi: 10.7506/spkx1002-6630-201210006

• 工艺技术 • 上一篇    下一篇

米糠蛋白提取中的关键影响因素及其优化

管 骁,金周筠,金 晶,黄成龙,金 龙   

  1. 上海理工大学医疗器械与食品学院
  • 出版日期:2012-05-25 发布日期:2012-05-07
  • 基金资助:
    国家自然科学基金青年科学基金项目(31101348;31000780);上海市晨光计划项目(09CG50)

Optimization of Key Factors for Protein Extraction from Rice Bran

GUAN Xiao,JIN Zhou-yun,JIN Jing,HUANG Cheng-long,JIN Long   

  1. (School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)
  • Online:2012-05-25 Published:2012-05-07

摘要: 研究米糠蛋白制备过程中存在的提取率低、产品颜色深等问题。在单因素试验的基础上,采用中心组合试验设计与响应面分析方法对提取温度、pH值、液料比以及辅助剂用量4因素进行优化,建立响应值(包括蛋白提取率与提取液色泽)与各影响因素之间的回归方程,得出最优提取条件为提取温度40.8℃、液料比12:1(mL/g)、pH10.5、辅助剂用量0.5%,此条件下米糠蛋白理论提取率为60.4%,提取液色泽理论值(L值)56.7;验证实验表明蛋白提取率59.5%,提取液色泽L值58.1,证明优化结果是可靠的。

关键词: 米糠蛋白, 提取, 响应面分析, 优化

Abstract: There usually are some shortcoming such as low protein extraction ratio and dark color during the rice bran protein extraction. The factors which caused these problems include extraction temperature, pH, ratio of solvent to material, and auxiliary agent amount. The central composite experiments and response surface analysis were used to optimize these factors. The experiments results showed that 60.4% protein extraction ratio and extraction supernatant color (L value) 56.7 were obtain under the optimized condition of temperature 40.8℃, ratio of solvent to material 12, pH 10.5, and auxiliary agent amount 0.5%. The actual protein extraction ratio was 59.5%, and L value 58.1, which showed that the optimized conditions were accurate.

Key words: rice bran protein, extraction, response surface analysis, optimization

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