食品科学 ›› 2012, Vol. 33 ›› Issue (12): 173-176.doi: 10.7506/spkx1002-6630-201212034

• 分析检测 • 上一篇    下一篇

普洱茶中真菌合成洛伐他汀的能力分析

赵振军1,童华荣2,周 黎2,刘勤晋2,*   

  1. 1.长江大学园艺园林学院 2.西南大学食品科学学院
  • 出版日期:2012-06-25 发布日期:2012-07-27
  • 基金资助:
    湖北省教育厅科学技术研究项目(Q20121208);云南省普洱茶关键技术研究项目(2007YNCXB-01-01)

Analysis of Lovastatin-Producing Ability of Fungi in Pu-erh Tea

ZHAO Zhen-jun1,TONG Hua-rong2,ZHOU Li2,LIU Qin-jin2,*   

  1. (1. College of Horticulture and Landscape Architecture, Yangtze University, Jingzhou 434025, China; 2. College of Food Science, Southwest University, Chongqing 400716, China)
  • Online:2012-06-25 Published:2012-07-27

摘要: 对从普洱茶中分离的曲霉、青霉和木霉属真菌进行纯培养,采用液相色谱法分析培养基中洛伐他汀的含量,鉴定普洱茶中真菌合成洛伐他汀的能力。结果显示:曲霉属真菌中1株塔宾曲霉、1株温特曲霉和1株烟曲霉具有合成洛伐他汀的能力,在察氏(CYA)培养基中检测到的洛伐他汀含量分别为(9.59±0.42)、(2.33±0.21)、(2.77±0.13)ng/g;青霉属真菌中只有产黄青霉在CYA培养基上能检测到洛伐他汀的存在,检测含量为(3.36±0.69)ng/g;而从普洱茶中分离到的2株木霉属真菌--棘孢木霉和橘绿木霉均具有合成洛伐他汀的能力,在CYA培养基中检测到的洛伐他汀的含量分别为(4.8±0.17)、(1.47±0.36)ng/g。

关键词: 普洱茶, 洛伐他汀, 真菌

Abstract: The pure strains of Aspergillus, Penicillium and Trichoderma isolated from Pu-erh tea were cultured individually to analyze their ability to produce lovastatin by HPLC. The results showed that only one strain of A. tubingensis, one strain of A. wenti and one strain of A. fumigatus among the genera of Aspergillus had the ability to synthesize lovastatin with the content of 9.59, 2.33 ng/g and 2.77 ng/g, respectively, after culture in the Czapek Yeast Agar (CYA) medium. On the CYA medium, only P. chrysogenum revealed the ability to secrete lovastatin with the content of 3.36 ng/g. Two strains of Trichoderma (T. asperellum and T. citrinoviride) from Pu-erh tea samples had the ability to produce lovastatin with the content of 4.8 ng/g and 1.47 ng/g on the CYA medium, respectively.

Key words: Pu-erh tea, lovastatin, fungi

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