食品科学 ›› 2012, Vol. 33 ›› Issue (12): 268-272.doi: 10.7506/spkx1002-6630-201212055

• 分析检测 • 上一篇    下一篇

电子鼻结合气相色谱- 质谱联用技术分析黄鳍金枪鱼肉的挥发性成分

王 霞1,黄 健1,侯云丹1,王求娟2,陈义方2,夏静波2,苏秀榕1,*   

  1. 1.宁波大学海洋学院 2.宁波今日食品有限公司
  • 出版日期:2012-06-25 发布日期:2012-07-27
  • 基金资助:
    宁波市科技局农业与社发重大科技项目(2010C10040)

Analysis of Volatile Components in Yellowfin Tuna by Electronic Nose and GC-MS

WANG Xia1,HUANG Jian1,HOU Yun-dan1,WANG Qiu-juan2,CHEN Yi-fang2,XIA Jing-bo2,SU Xiu-rong1,*   

  1.  (1. School of Marine Sciences, Ningbo University, Ningbo 315211, China; 2. Ningbo Today Food Co. Ltd., Ningbo 315502, China)
  • Online:2012-06-25 Published:2012-07-27

摘要: 目的:探索黄鳍金枪鱼肉挥发性成分与加工温度的关系。方法:利用电子鼻分别检测不同温度(70、80、90、100、120、150℃)加热条件下的黄鳍金枪鱼肉及其生肉样品,并对所获数据进行主成分分析,在此基础上进一步采用顶空固相微萃取和气相色谱-质谱联用技术对新鲜和分别加热到95、150℃的黄鳍金枪鱼肉样品进行分析。结果:不同条件下的样品电子鼻信号表现出很强的聚类特性。经气相色谱-质谱联用技术测定3种样品,分别检出52、50、53种成分,主要为醛类、烃类、醇类、酮类、呋喃类,它们的协同作用构成了黄鳍金枪鱼肉特有的特征气味。结论:黄鳍金枪鱼生肉中含有较多的腥味成分--1-戊烯-3-醇、1,5-辛二烯-3-醇,通过加热处理后这两种成分的相对含量明显减少;加热处理后,具有特殊香气的长链醛以及呋喃类物质相对含量明显增多,2-乙基呋喃、2-戊基呋喃是烤制肉品重要的香味呈味物,是加热后黄鳍金枪鱼的特征气味。

关键词: 黄鳍金枪鱼, 电子鼻, 气相色谱-质谱联用, 挥发性成分

Abstract: Purpose: To discover the effect of processing temperature on the volatile composition of yellowfin tuna. Methods: An electronic nose was used to detect raw and thermally processed (70, 80, 90, 100, 120 ℃ and 150 ℃) yellowfin tuna samples. The data obtained were analyzed by principal component analysis (PCA). Moreover, raw, 95 ℃ processed and 150 ℃ processed yellowfin tuna samples were analyzed by headspace solid-phase micro-extraction and GC-MS. Results: The electronic nose signals of different samples showed a strong cluster property. A total of 52, 50 and 53 volatile compounds were identified in aw, 95 ℃ processed and 150 ℃ processed yellowfin tuna samples, respectively. Among these compounds, the predominant ones were aldehydes, hydrocarbons, alcohols, kotones and furans, which synergistically contributed to the characteristic flavor of yellowfin tuna. Conclusions: Raw yellowfin tuna contains a large amount of the off-flavor compounds 1-penten-3-ol and 1,5-octadien-3-ol. Thermal processing can result in a significant decrease in their relative contents but a significant increase in the contents of the aroma-active compounds long-chain aldehydes and furans. 2-Ethyl-furan and 2-pentyl-furan are responsible for the characteristic aroma of roast yellowfin tuna.

Key words: yellow fin tuna, electronic nose, gas chromatography-mass spectrometry (GC-MS), volatile components

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