关键词: 小龙虾, 优势腐败菌, 培养基法, 高通量测序

Abstract: In order to reveal the microbial diversity and specific spoilage organisms (SSOs) of red swamp crayfish under refrigerated condition (4 ℃), we individually isolated the SSOs using the culture-dependent method in this study and evaluated their spoilage abilities. Meanwhile, high-throughput sequencing technology was used to identify the spoilage bacterial species in fresh crayfish and those at the middle and late stages of spoilage and to explore the changes in the microbial community during refrigerated storage. The results showed that a higher bacterial diversity was detected by highthroughput sequencing compared to the culture-dependent method and the former method identified more genera than the latter. A total of 11 strains of SSOs were detected by the culture-dependent method, among which Aeromonas jandaei had the strongest spoilage ability while Citrobacter freundii had the weakest spoilage ability. In addition, high-throughput sequencing revealed that the total relative abundance of Shewanella sp., Carnobacterium sp., Brochothrix sp., Psychrobacter sp., Vagococcus sp. and Acinetobacter sp. was 90.57% at the end of shelf life. By comparing the results of the two methods, it was found that the SSOs in chilled crayfish mainly included Shewanella sp., Carnobacterium sp., Brochothrix sp., Psychrobacter sp., Vagococcus sp., Acinetobacter sp., Aeromonas jandaei, and Trichococcus sp..

Key words: red swamp crayfish, specific spoilage organism, culture-dependent method, high-throughput sequencing