食品科学 ›› 2019, Vol. 40 ›› Issue (20): 217-226.doi: 10.7506/spkx1002-6630-20181102-024

• 成分分析 • 上一篇    下一篇

基于代谢组学技术的玛咖产地鉴别

张宏蕊,张九凯,韩建勋,邢冉冉,郑磊,陈颖   

  1. (1.中国检验检疫科学研究院,北京 100176;2.合肥工业大学食品与生物工程学院,安徽 合肥 230009)
  • 出版日期:2019-10-25 发布日期:2019-10-25
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFF0211004)

ZHANG Hongrui, ZHANG Jiukai, HAN Jianxun, XING Ranran, ZHENG Lei, CHEN Ying

ZHANG Hongrui, ZHANG Jiukai, HAN Jianxun, XING Ranran, ZHENG Lei, CHEN Ying   

  1. (1. Chinese Academy of Inspection and Quarantine, Beijing 100176, China;2. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China)
  • Online:2019-10-25 Published:2019-10-25

摘要: 采用代谢组学的方法,基于超高效液相色谱-四极杆串联飞行时间质谱对不同产地的玛咖进行产地鉴别和各产地代谢物差异分析。通过正交偏最小二乘判别分析成功区分玛咖的5 个产地,并对各个产地玛咖的代谢物进行靶标和非靶标代谢组学分析,其中靶标代谢组学分析共鉴定出28 种代谢物,包括玛咖酰胺、玛咖烯、芥子油苷、生物碱、甾醇、多酚和黄酮,非靶标代谢组学分析鉴定出玛咖的产地标志物。结果表明代谢组学方法可以用于玛咖产地鉴别以及全组分的鉴定。

关键词: 玛咖, 代谢组学, 超高效液相色谱-四极杆串联飞行时间质谱, 产地鉴别, 化学计量学

Abstract: A metabolomics method was proposed for identifying the geographical origin of Maca and the differences in metabolites among geographical origins were analyzed by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Five origins of Maca were successfully distinguished by orthogonal partial least squares discriminant analysis and then the target and non-target metabolites of Maca from each geographical origin were analyzed. A total of 28 metabolites, including macaamide, macaene, glucosinolate, alkaloids, sterols, polyphenols and flavonoids, were identified by target metabolomics analysis. The markers from Maca geographical origin discrimination were identified by non-target metabolomics analysis. It is concluded that the metabolomics method can be used for the discrimination of Maca origin and the identification of the whole component.

Key words: Maca, metabolomics, ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry, geographical origin discrimination, chemometics

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