桑葚多糖对镉源性肝损伤小鼠的保护作用

doi:10.7506/spkx1002-6630-20210131-372

食品科学 ›› 2022, Vol. 43 ›› Issue (1): 135-141.doi: 10.7506/spkx1002-6630-20210131-372

• 营养卫生 • 上一篇

桑葚多糖对镉源性肝损伤小鼠的保护作用

李变丽，陈华国，赵超，龚小见，周欣

（1.贵州中医药大学药学院，贵州贵阳 550002；2.贵州师范大学喀斯特山地生态环境保护与资源利用协同创新中心，贵州贵阳 550001；3.贵州师范大学贵州省药物质量控制及评价技术工程实验室，贵州贵阳 550001）

发布日期:2022-01-29
基金资助:
国家自然科学基金面上项目（31570358）；贵州省科技计划项目（黔科合支撑［2018］2826）；贵州省高层次创新人才培养项目（黔科合人才（2015）4033号）

Protective Effect of Mori Fructus Polysaccharide on Liver Injury Induced by Cadmium in Mice

LI Bianli, CHEN Huaguo, ZHAO Chao, GONG Xiaojian, ZHOU Xin

(1. School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550002, China; 2. Collaborative Innovation Center for Karst Mountain Ecological Environment Protection and Resource Utilization, Guizhou Normal University, Guiyang 550001, China; 3. Guizhou Provincial Laboratory of Drug Quality Control and Evaluation Technology, Guizhou Normal University, Guiyang 550001, China)

Published:2022-01-29

摘要/Abstract

摘要： 目的：探究桑葚多糖（Mori Fructus polysaccharide，MFP）对镉致肝损伤小鼠的保护作用。方法：将雄性KM小鼠随机分成空白组、模型组、阳性组及MFP组，用氯化镉（CdCl2）溶液染毒，分别建立小鼠急性期及慢性期肝损伤模型。观测不同时期小鼠的肝脏指数、血常规指标水平、肝匀浆中谷草转氨酶（asparate aminotransferase，AST）、谷丙转氨酶（alanine aminotransferase，ALT）、碱性磷酸酶（alkaline phosphatase，ALP）水平及肝脏病理变化，以评价MFP的治疗效果。结果：在小鼠急性肝损伤模型中（1～7?d），与模型组相比，MFP组肝匀浆中AST、ALT、ALP活力分别显著降低33.32%、33.38%、34.11%（P＜0.05），且能明显改善肝脏病理变化；在小鼠慢性肝损伤模型中（1～28?d），与模型组相比，MFP组肝损伤小鼠的肝脏指数极显著降低21.20%（P＜0.01），且MFP组能极显著降低肝匀浆中AST、ALT、ALP活力（P＜0.01），分别降低27.01%、33.46%、29.69%，并致小鼠血液中红细胞平均血红蛋白质量浓度显著升高4.7%（P＜0.05）。结论：MFP对镉源性肝损伤具有保护作用，该研究可为MFP用于镉源性肝损伤的防治提供参考依据。

关键词: 桑葚多糖；肝损伤；氯化镉；保护作用

Abstract: Objective: To explore the protective effect of Mori Fructus polysaccharide (MFP) on cadmium-induced liver injury in mice. Methods: Male Kunming mice were randomly divided into blank, model, positive control and MFP groups. Cadmium chloride (CdCl2) solution was intraperitoneally injected into mice for up to seven and 28 days to establish acute and chronic liver injury models, respectively. The liver index, routine blood parameters, the activity levels of asparate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels in liver homogenate, and pathological changes in the liver were observed to evaluate the therapeutic effect of MFP. Results: Compared with the acute liver injury model group, the activities of hepatic AST, ALT and ALP in the MFP group was decreased by 33.32%, 33.38% and 34.11%, respectively (P < 0.05), and the pathological changes in the liver were significantly improved. Compared with the chronic liver injury model group, MFP reduced the liver index of mice with liver injury by 21.20% (P < 0.01), decreased the activities of AST, ALT and ALP in liver homogenate by 27.01%, 33.46% and 29.69%, respectively (P < 0.01), and increased the mean corpuscular hemoglobin concentration (MCHC) by 4.7% (P < 0.05). Conclusion: MFP has a protective effect on cadmium-induced liver injury. This finding provides evidence that MFP can be used in the prevention and treatment of cadmium-induced liver injury.

Key words: Mori Fructus polysaccharide; liver injury; cadmium chloride; protective effect

中图分类号:

TS201.4

李变丽，陈华国，赵超，龚小见，周欣. 桑葚多糖对镉源性肝损伤小鼠的保护作用[J]. 食品科学, 2022, 43(1): 135-141.

LI Bianli, CHEN Huaguo, ZHAO Chao, GONG Xiaojian, ZHOU Xin. Protective Effect of Mori Fructus Polysaccharide on Liver Injury Induced by Cadmium in Mice[J]. FOOD SCIENCE, 2022, 43(1): 135-141.

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桑葚多糖对镉源性肝损伤小鼠的保护作用

Protective Effect of Mori Fructus Polysaccharide on Liver Injury Induced by Cadmium in Mice

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