沙枣胶/枯茗醛复合涂膜对番茄采后红粉病的抑制作用及机制

摘要/Abstract

摘要： 摘要：红粉病是番茄果实主要的病害之一，采前采后均可发生，给番茄生产和贮运造成了较大损失。本试验研究枯茗醛对番茄红粉病病原真菌粉红单端孢（Trichothecium roseum）的体外抑制活性，并制备沙枣胶与枯茗醛复合涂膜‘千禧’樱桃番茄，果实经损伤接种后探究该涂膜对番茄红粉病的抑制作用及其对番茄果实抗性的影响。结果显示，枯茗醛在离体条件下能有效抑制T. roseum孢子萌发和菌丝生长，在0.1 μL/mL浓度作用下，对菌落生长的抑制率达80%以上。在体内条件下，沙枣胶与枯茗醛复合涂膜能明显抑制果实上T. roseum菌体生长，延缓病斑扩展并降低番茄果实接种发病率，在贮藏第9 d，30 mg/mL沙枣胶与体积分数0.2%枯茗醛复合涂膜组病斑直径和发病率分别为对照组的36.7%和69.2%。复合涂膜可显著提高番茄果实接种前期H2O2含量，并显著提高果实贮藏期间过氧化物酶、苯丙氨酸解氨酶和4-香豆酸辅酶A连接酶的酶活性，促进果实中总酚和类黄酮的积累。沙枣胶与枯茗醛复合涂膜能够有效抑制番茄采后红粉病的发生与发展，其作用机制不仅与枯茗醛抑制病原菌生长有关，还与诱导番茄果实增强抗性有关。

关键词: 关键词：番茄, 红粉病, 沙枣胶, 枯茗醛, 抑制作用, 抗性

Abstract: Abstract: Pink-rot is one of the main diseases of tomatoes which can occur before and after harvest, causing great losses to tomato production, storage and transportation. This study investigated the inhibitory ability of cuminal alone and in combination with oleaster gum against Trichothecium roseum in vitro and in vivo assay respectively. Moreover, explored the inhibitory effect of the composite coating on postharvest pink rot of tomatoes and its effect on the resistance of tomato fruits. The results showed that cuminal effectively inhibited the spore germination and mycelial growth of T. roseum under in vitro conditions. At a concentration of 0.1 μL/mL, the inhibitory rate of the colony growth was more than 80%. Under in vivo conditions, the composite coating significantly inhibited the growth of T. roseum on the fruit, delayed the lesion expansion and reduced the decay incidence of tomato fruits. After 9 d of storage, the lesion diameter and the decay incidence of the fruit treated with the composite coating containing oleaster gum and 0.2% cuminal were 36.7% and 69.2% of those in the control group, respectively. The composite coating effectively increased the H2O2 content of tomato fruits during the early stage of storage, increased the enzyme activities such as peroxidase, phenylalanine ammonia-lyase and 4-coumarate-CoA ligase, and enhanced the accumulation of total phenols and flavonoids in the fruit. The results suggest that the composite coating containing oleaster gum and cuminal can effectively inhibit the occurrence and development of postharvest pink rot of tomatoes, and its mechanism is not only related to the inhibition of pathogen growth, but also to the induction of enhanced resistance to tomato fruits.

Key words: Key words: tomato, pink rot, oleaster gum, cuminal, inhibitory effect, resistance

中图分类号:

TS255.3

戴希爽张忠毕阳 William Oyom 张志鹏谢鹏东. 沙枣胶/枯茗醛复合涂膜对番茄采后红粉病的抑制作用及机制[J]. 食品科学, 0, (): 0-0.

Yang BI. Inhibitory Effect and Mechanism of Composite Coating with Oleaster Gum and Cuminal on Postharvest Pink Rot of Tomatoes[J]. FOOD SCIENCE, 0, (): 0-0.

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