薏苡仁生物肽对高糖损伤人脐静脉内皮细胞保护作用

摘要/Abstract

摘要： 研究薏苡仁生物肽对高糖损伤人脐静脉内皮细胞（Human Umbilical Vein Endothelial Cells，HUVEC）的保护作用及机制。采用30 mM葡萄糖建立HUVEC高糖损伤模型，不同浓度薏苡仁生物肽作用于高糖损伤HUVEC后， MTT法检测细胞活力；硝酸还原酶法检测细胞NO分泌量；流式细胞仪检测细胞活性氧（Reactive Oxygen Species，ROS）含量；Annexin V-FITC/PI双染检测细胞凋亡和坏死情况；实时荧光定量PCR检测炎症相关基因表达变化。结果表明，高糖损伤导致HUVEC NO分泌量降低28.72%（P ＜ 0.05），ROS分泌增加96.19%（P ＜ 0.05）， ACE基因表达量显著降低，ICAM-1、IL-6、MCP-1和VCAM基因表达量显著增加；随着高糖作用时间延长， HUVEC凋亡和坏死细胞比例显著增加，为NG组的1.61倍。薏苡仁生物肽均不同程度增加NO分泌量，降低ROS含量，逆转了高糖损伤导致的ACE基因表达量降低，使炎症基因表达量显著降低，减少细胞凋亡。因而，薏苡仁生物肽对高糖损伤HUVEC具有一定的保护作用。

关键词: 薏苡仁生物肽, 高糖, 人脐静脉内皮细胞, 氧化应激, 炎症

Abstract: To investigate the protective effect and mechanisms of coix biopeptide on high glucose injured human umbilical vein endothelial Cells (HUVEC). The injury model of HUVEC was established by the treating the HUVEC with 30 mM glucose, and then the protection effect of the Coix biopeptide was evaluated. Cell viability was detected by MTT assay. Nitric acid reductase was used to detect NO secretion. The content of reactive oxygen species (ROS) was detected by flow cytometry. Apoptosis and necrosis were detected by Annexin V-FITC/PI double staining. Quantitative real-time PCR was applied to detect the expression of inflammation-related genes. The results showed that NO secretion decreased by 28.72% (P < 0.05), ROS secretion increased by 96.19% (P < 0.05), the expression of ACE gene was significantly decreased, and the expression of ICAM-1, IL-6, MCP-1 and VCAM genes was significantly increased in HUVEC induced by high glucose injury. With the prolongation of high glucose treatment, the proportion of apoptotic and necrosis cells in HUVEC increased significantly, which was 1.61 times of that in NG group. Coix biopeptides increased NO secretion and decreased ROS content to varying degrees, reversed the decrease in ACE gene expression caused by high glucose injury, significantly reduced the expression of inflammatory genes and reduced cell apoptosis. In summary, Coix biopeptide possessed explicit protection effect on by high glucose injured HUVEC.

Key words: Coix biopeptide, high glucose, human umbilical vein endothelial cells, oxidative stress, inflammation

尹艳燕赵艺褚辉程梁劲杰韩晓佳陈祖君王灵芝. 薏苡仁生物肽对高糖损伤人脐静脉内皮细胞保护作用[J]. 食品科学, 0, (): 0-0.

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