原花青素B2对高脂膳食小鼠钙代谢及股骨基因表达谱的影响

doi:10.7506/spkx1002-6630-20211007-050

摘要/Abstract

摘要： 目的：探究原花青素B2对高脂膳食小鼠钙代谢异常与股骨基因表达的影响。方法：将27 只C57BL/6雄性小鼠随机分为3 组：对照组、高脂膳食组和原花青素B2组，原花青素B2添加量为0.2%（以饲料质量计）；持续饲喂8 周后分析小鼠体质量、血浆脂质水平、钙内稳态、钙吸收能力和骨矿物含量的变化，并运用真核有参转录组技术分析小鼠股骨基因表达变化情况。结果：原花青素B2能够显著提高小鼠钙净吸收和贮留能力（P＜0.05），并显著降低血脂水平和甲状旁腺素质量浓度，改善高脂膳食导致的小鼠骨矿物、股骨钙含量和最大荷载量的降低。高通量测序发现49 个差异共表达基因，原花青素B2可干预其中48 个基因的表达，基因本体论富集分析发现其归属于生物进程和细胞组分两个分支，京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）富集分析发现共有13 条通路具有显著性富集意义（P＜0.05），其中涉及钙代谢相关信号转导和代谢通路5 个基因（Cdkn2a、Adrb1、Fcgr4、Aldh1a3和Calml3）的表达量发生显著改变（P＜0.05）。与高脂膳食组相比，原花青素B2下调Cdkn2a、Adrb1、Fcgr4基因表达量分别为9.19、3.68 倍和3.61 倍，上调Aldh1a3和Calml3基因的表达量分别为2.06 倍和3.29 倍。结论：原花青素B2具有干预高脂膳食导致的小鼠钙代谢异常的作用，其可能与下调破骨细胞活性与上调成骨细胞活性相关基因的表达有关。

关键词: 原花青素B2；钙代谢异常；转录组；基因表达

Abstract: Objective: To investigate the effect of procyanidin B2 on gene expression in the femur of high-fat diet (HFD)-fed mice with calcium metabolism disorders. Methods: Twenty-seven C57BL/6 male mice were randomly divided into three groups: control, HFD, and HFD plus 0.2% procyanidin B2. The body mass, plasma lipid levels, calcium homeostasis, calcium absorption capacity, and bone mineral levels of the mice were measured after eight weeks of feeding, and gene expression in the femur of the mice was determined by using eukaryotic transcriptome sequencing. The results showed that procyanidin B2 significantly reduced plasma lipid and parathyroid hormone levels, significantly increased net calcium absorption and storage capacity (P < 0.05), and ameliorated the reduction in bone mineral, femoral calcium content, and maximum loading capacity in HFD-fed mice. Forty-nine differentially co-expressed genes (DCGs) were identified by high-throughput sequencing, and procyanidin B2 could improve the expression of 48 of these genes. Gene ontology (GO) enrichment analysis showed that these DCGs belonged to the two branches of biological processes and cell components. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that 13 pathways were significantly enriched (P < 0.05). The expression levels of five DCGs, Cdkn2a, Adrb1, Fcgr4, Aldh1a3, and Calml3, involved in the calcium metabolism-related signal transduction and metabolic pathways were significantly changed (P < 0.05). Compared with the HFD group, procyanidin B2 supplementation down-regulated the expression of the Cdkn2a, Adrb1, and Fcgr4 genes by 9.19-, 3.68-, and 3.61-fold, and up-regulated the expression of the Aldh1a3 and Calml3 genes by 2.06- and 3.29-fold, respectively. Conclusion: Procyanidin B2 ameliorates calcium metabolism disorders in HFD-fed mice, which may be associated with the down-regulated expression of genes related to osteoblast activity and up-regulated expression of genes related to osteoclast activity.

Key words: procyanidin B2; calcium metabolism disorders; transcriptome; gene expression

中图分类号:

TS201.4

张方方，肖瀛，杨昌铭，吴其国，周一鸣，周小理. 原花青素B2对高脂膳食小鼠钙代谢及股骨基因表达谱的影响[J]. 食品科学, 2022, 43(21): 144-153.

ZHANG Fangfang, XIAO Ying, YANG Changming, WU Qiguo, ZHOU Yiming, ZHOU Xiaoli. Effect of Procyanidin B2 on Calcium Metabolism and Femur Gene Expression Profile in High-Fat Diet Fed Mice[J]. FOOD SCIENCE, 2022, 43(21): 144-153.

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原花青素B2对高脂膳食小鼠钙代谢及股骨基因表达谱的影响

Effect of Procyanidin B2 on Calcium Metabolism and Femur Gene Expression Profile in High-Fat Diet Fed Mice

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