不同蛋黄油组分促进小鼠消化性胃溃疡愈合作用

doi:10.7506/spkx1002-6630-20211204-047

摘要/Abstract

摘要： 目的：比较蛋黄油中分离的4 种脂质组分（分别记为蛋黄油1、蛋黄油2、蛋黄油3及磷脂）促进小鼠消化性胃溃疡愈合的效果。方法：对6 周龄胃溃疡模型小鼠分别灌胃4 种蛋黄油脂质组分（0.3 g/d蛋黄油、0.1 g/d磷脂），干预5 d后以胃窦溃疡情况、组织病理学分析结果及胃组织生化指标（超氧化歧化酶（superoxide dismutase，SOD）、丙二醛（malondialdehyde，MDA）、谷胱甘肽过氧化酶（glutathione peroxidase，GSH-Px）、内皮素1（endothelin-1，ET-1）、一氧化氮（NO）水平）评价不同脂质组分促进小鼠消化性胃溃疡愈合的效果；进一步对胃溃疡模型小鼠分别灌胃高剂量（0.3 g/d）、低剂量（0.15 g/d）蛋黄油1以及高剂量（0.1 g/d）、低剂量（0.05 g/d）磷脂进行验证性实验。结果：蛋黄油1和磷脂作用胃组织后，小鼠胃窦溃疡明显愈合，组织病理学分析结果表明4 种蛋黄油脂质组分干预可以维持细胞完整性并抑制炎性细胞浸润。与模型对照组相比，蛋黄油1干预后胃组织SOD、GSH-Px、NO水平显著或极显著提升（P＜0.05、P＜0.01），MDA和ET-1含量分别极显著和显著下降（P＜0.01、P＜0.05）；磷脂干预后胃组织SOD活力与GSH-Px含量极显著提升（P＜0.01），MDA、ET-1含量极显著或显著降低（P＜0.01、P＜0.05）；验证性实验结果表明，低剂量（0.15 g/d）蛋黄油1干预对小鼠消化性胃溃疡仍有显著促愈合效果，但低剂量（0.05 g/d）磷脂干预效果不明显。结论：较高n-3不饱和脂肪酸和花生四烯酸占比的液态脂质（蛋黄油1）及磷脂为蛋黄油中促进消化性胃溃疡愈合的有效成分，且对胃溃疡模型小鼠灌胃0.15 g/d蛋黄油1或0.1 g/d磷脂均有良好的促进消化性胃溃疡愈合效果。

关键词: 蛋黄油；消化性胃溃疡；抗溃疡作用；脂质分离

Abstract: Objective: To compare the effects of four lipid components (oil fractions 1, 2 and 3, and phospholipids) extracted from egg yolk oil on promoting peptic gastric ulcer healing in mice. Methods: The lipid components at 0.3 g/d for the oil fractions and 0.1 g/d for phospholipids were separately given to six-week-old mice with gastric ulcer by gavage for five days. Afterwards, the extent of gastric antrum ulcer and histopathological alterations were analyzed, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px), malondialdehyde (MDA), endothelin-1 (ET-1) and nitric oxide (NO) levels in gastric tissue were measured to evaluate the promoting-healing effect of the lipid components on peptic gastric ulcer. Furthermore, verification experiments were carried out by gavaging the mice with gastric ulcer with oil fraction 1 at doses of 0.3 and 0.15 g/d or phospholipids at doses of 0.1 and 0.05 g/d. Results: Yolk oil 1 and phospholipids significantly promoted the healing of gastric antrum ulcer. Histopathological analysis showed the four lipid components maintained cell integrity and inhibited inflammatory cell infiltration. Compared with the model group, SOD activity and GSH-Px and NO levels were significantly increased (P < 0.05 and P < 0.01), and MDA and ET-1 levels were significantly decreased after intervention with oil fraction 1 (P < 0.01 and P < 0.05). Similarly, after phospholipid treatment, SOD activity and GSH-Px levels were significantly increased (P < 0.01), and MDA and ET-1 levels were significantly decreased (P < 0.01 and P < 0.05). In the validation experiments, the low dose of oil fraction 1 could still significantly promote ulcer healing, while the dose of phospholipids could not. Conclusion: Liquid lipids (yolk oil 1) rich in n-3 polyunsaturated fatty acids (PUFAs) and arachidonic acid as well as phospholipids in egg yolk are effective in promoting the healing of peptic gastric ulcer in mice, and oral administration of 0.15 g/d of yolk oil 1 or 0.1 g/d of phospholipids can promote the healing of peptic gastric ulcer in mice.

Key words: egg yolk oil; peptic gastric ulcer; anti-ulcer effect; lipids isolated

中图分类号:

TS201.4

田笑，仝其根. 不同蛋黄油组分促进小鼠消化性胃溃疡愈合作用[J]. 食品科学, 2022, 43(21): 163-170.

TIAN Xiao, TONG Qigen. Effect of Different Lipid Components from Egg Yolk Oil on Promoting the Healing of Peptic Gastric Ulcer in Mice[J]. FOOD SCIENCE, 2022, 43(21): 163-170.

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不同蛋黄油组分促进小鼠消化性胃溃疡愈合作用

Effect of Different Lipid Components from Egg Yolk Oil on Promoting the Healing of Peptic Gastric Ulcer in Mice

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