生物传感器在新烟碱类农药残留检测中的应用

摘要/Abstract

摘要： 新烟碱类杀虫剂在食品和环境样品中的超标残留严重威胁人体健康，提高新烟碱类农药残留的检测水平刻不容缓。基于生物传感器的新烟碱类农药残留物检测平台，可以实现对农药残留的快速、简单和低成本检测。本综述主要总结了电化学传感器、荧光传感器、比色传感器、电化学发光生物传感器和拉曼化学生物传感器在新烟碱类农药残留快速检测中的最新研究进展。同时，对其面临的挑战和解决方案进行了探讨，为日后生物传感器在农药残留检测方面的进一步开发和应用提供参考。

关键词: 新烟碱类农药, 残留检测, 生物传感器, 检测技术, 应用

Abstract: The excessive residue of neonicotinoid insecticides in food and environmental samples poses a serious threat to human health, and it is urgent to improve the detection level of neonicotinoid pesticide residues. A new nicotine pesticide residue detection platform based on biosensors can achieve fast, simple, and low-cost detection of pesticide residues. This review mainly summarizes the latest research progress of electrochemical sensors, fluorescence sensors, colorimetric sensors, electrochemiluminescence biosensors, and Raman chemical biosensors in the rapid detection of neonicotinoid pesticide residues. At the same time, it explores the challenges and solutions they face, providing reference for the further development and utilization of biosensors in pesticide residue detection in the future.

Key words: Neonicotinoid pesticides (NEOs), pesticide residue detection, Biosensors, detection technology, application

中图分类号:

TS207.3

李瑶涂济源孙忠月罗可馨. 生物传感器在新烟碱类农药残留检测中的应用[J]. 食品科学.

Yao Li Zhong-Yue SUN. Research Advances in biosensors for the Detection of Neonicotinoid Pesticide Residues[J]. FOOD SCIENCE.

参考文献

[1]XU L, ABD EL-ATY A M, EUN J B, et al.Recent Advances in Rapid Detection Techniques for Pesticide Residue: A Review[J].Journal of agricultural and food chemistry, 2022, 70(41):13093-117 [2]GOH M S, LAM S D, YANG Y, et al.Omics technologies used in pesticide residue detection and mitigation in crop [J]. Journal of hazardous materials, 2021, 420: 126624. DOI: 10.1016/j.jhazmat.2021.126624. [3]NARENDERAN S T, MEYYANATHAN S N, BABU B.Review of pesticide residue analysis in fruits and vegetables. Pre-treatment, extraction and detection techniques [J]. Food research international (Ottawa, Ont), 2020, 133: 109141. DOI: 10.1016/j.foodres.2020.109141. [4]BASS C, DENHOLM I, WILLIAMSON M S, et al.The global status of insect resistance to neonicotinoid insecticides [J]. Pesticide biochemistry and physiology, 2015, 121: 78-87. DOI: 10.1016/j.pestbp.2015.04.004. [5]WANG Y, QIN J, LU Q, et al.Residue detection and correlation analysis of multiple neonicotinoid insecticides and their metabolites in edible herbs [J]. Food Chem X, 2023, 17: 100603. DOI: 10.1016/j.fochx.2023.100603. [6]LIU Q, WANG Q, XU C, et al.Organochloride pesticides impaired mitochondrial function in hepatocytes and aggravated disorders of fatty acid metabolism [J]. Scientific reports, 2017, 7: 46339. DOI: 10.1038/srep46339. [7]HE B, WANG X, YANG C, et al.The regulation of autophagy in the pesticide-induced toxicity: Angel or demon? [J]. Chemosphere, 2020, 242: 125138. DOI: 10.1016/j.chemosphere.2019.125138. [8]NIE J, SUN Y, ZHOU Y, et al.Bioremediation of water containing pesticides by microalgae: Mechanisms, methods, and prospects for future research [J]. The Science of the total environment, 2020, 707: 136080. DOI: 10.1016/j.scitotenv.2019.136080. [9]HAN W, TIAN Y, SHEN X.Human exposure to neonicotinoid insecticides and the evaluation of their potential toxicity: An overview [J]. Chemosphere, 2018, 192: 59-65. DOI: 10.1016/j.chemosphere.2017.10.149. [10]LI A J, MARTINEZ-MORAL M P, KANNAN K.Variability in urinary neonicotinoid concentrations in single-spot and first-morning void and its association with oxidative stress markers [J]. Environment international, 2020, 135: 105415. DOI: 10.1016/j.envint.2019.105415. [11]VUONG A M, ZHANG C, CHEN A.Associations of neonicotinoids with insulin and glucose homeostasis parameters in US adults: NHANES 2015-2016[J].Chemosphere, 2022, 286(Pt 1):131642- [12]ZHANG H, ZHANG R, ZENG X, et al.Exposure to neonicotinoid insecticides and their characteristic metabolites: Association with human liver cancer [J]. Environmental research, 2022, 208: 112703. DOI: 10.1016/j.envres.2022.112703. [13]QIN J A, FU Y, LU Q, et al.Matrix-matched monitoring ion selection strategy for improving the matrix effect and qualitative accuracy in pesticide detection based on UFLC-ESI-MS/MS: A case of Chrysanthemum [J]. Microchemical Journal, 2021, 160. DOI: 10.1016/j.microc.2020.105681. [14]LI Y, LUO Y, JIANG J, et al.Residual behavior and risk assessment of fluopyram, acetamiprid and chlorantraniliprole used individually or in combination on strawberry [J]. Environmental science and pollution research international, 2023. DOI: 10.1007/s11356-023-26544-x. [15]WEI X, PAN Y, TANG Z, et al.Neonicotinoids residues in cow milk and health risks to the Chinese general population [J]. Journal of hazardous materials, 2023, 452: 131296. DOI: 10.1016/j.jhazmat.2023.131296. [16]CASTRO J, SáNCHEZ-BRUNETE C, TADEO J L.Multiresidue analysis of insecticides in soil by gas chromatography with electron-capture detection and confirmation by gas chromatography-mass spectrometry[J].Journal of chromatography A, 2001, 918(2):371-80 [17]TOLEDANO R M, CORTéS J M, ANDINI J C, et al.Large volume injection of water in gas chromatography–mass spectrometry using the Through Oven Transfer Adsorption Desorption interface: Application to multiresidue analysis of pesticides[J].Journal of chromatography A, 2010, 1217(28):4738-42 [18]KRISTENSON E M, HAVERKATE E G, SLOOTEN C J, et al.Miniaturized automated matrix solid-phase dispersion extraction of pesticides in fruit followed by gas chromatographic-mass spectrometric analysis[J].Journal of chromatography A, 2001, 917(1-2):277-86 [19]SCHELLIN M, HAUSER B, POPP P.Determination of organophosphorus pesticides using membrane-assisted solvent extraction combined with large volume injection-gas chromatography-mass spectrometric detection[J].Journal of chromatography A, 2004, 1040(2):251-8 [20]HERNáNDEZ F, SANCHO J V, POZO O J.Critical review of the application of liquid chromatographymass spectrometry to the determination of pesticide residues in biological samples[J].Analytical and bioanalytical chemistry, 2005, 382(4):934-46 [21]SONGA E A, OKONKWO J O.Recent approaches to improving selectivity and sensitivity of enzyme-based biosensors for organophosphorus pesticides: A review [J]. Talanta, 2016, 155: 289-304. DOI: 10.1016/j.talanta.2016.04.046. [22]MURUGAIYAN S B, RAMASAMY R, GOPAL N, et al.Biosensors in clinical chemistry: An overview [J]. Advanced biomedical research, 2014, 3: 67. DOI: 10.4103/2277-9175.125848. [23]PéREZ-FERNáNDEZ B, MERCADER J V, ABAD-FUENTES A, et al.Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes [J]. Talanta, 2020, 209: 120465. DOI: 10.1016/j.talanta.2019.120465. [24]FAN L, ZHAO G, SHI H, et al.A highly selective electrochemical impedance spectroscopy-based aptasensor for sensitive detection of acetamiprid [J]. Biosensors & bioelectronics, 2013, 43: 12-8. DOI: 10.1016/j.bios.2012.11.033. [25]SUN Y, LI Z, HUANG X, et al.A nitrile-mediated aptasensor for optical anti-interference detection of acetamiprid in apple juice by surface-enhanced Raman scattering [J]. Biosensors & bioelectronics, 2019, 145: 111672. DOI: 10.1016/j.bios.2019.111672. [26]SABERI Z, REZAEI B, ENSAFI A A.Fluorometric label-free aptasensor for detection of the pesticide acetamiprid by using cationic carbon dots prepared with cetrimonium bromide[J].Mikrochimica acta, 2019, 186(5):273- [27]YANG L, SUN H, WANG X, et al.An aptamer based aggregation assay for the neonicotinoid insecticide acetamiprid using fluorescent upconversion nanoparticles and DNA functionalized gold nanoparticles[J].Mikrochimica acta, 2019, 186(5):308- [28]QI H, LI H, LI F.N-heterocyclic Ir(III) complex targeting G-quadruplex structure to boost label-free and immobilization-free electrochemiluminescent sensing [J]. Biosensors & bioelectronics, 2023, 220: 114839. DOI: 10.1016/j.bios.2022.114839. [29]SUN Y, ZHANG N, HAN C, et al.Competitive immunosensor for sensitive and optical anti-interference detection of imidacloprid by surface-enhanced Raman scattering [J]. Food chemistry, 2021, 358: 129898. DOI: 10.1016/j.foodchem.2021.129898. [30]ASAD M, ZULFIQAR A, RAZA R, et al.Orange Peel Derived C‐dots Decorated CuO Nanorods for the Selective Monitoring of Dopamine from Deboned Chicken[J].Electroanalysis, 2019, 32(1):11-8 [31]ONDER S, VAN GROL M, FIDDER A, et al.Rabbit Antidiethoxyphosphotyrosine Antibody,Made by Single B Cell Cloning,Detects Chlorpyrifos Oxon-Modified Proteins in Cultured Cells and Immunopurifies Modified Peptides for Mass Spectrometry[J].Journal of proteome research, 2021, 20(10):4728-45 [32]< 基于表面增强拉曼光谱标记技...用于农药残留检测的研究进展_颜朦朦.pdf> [J]. DOI: 10.7506/spkx1002-6630-20210908-092. [33]XU X, GUO Y, WANG L, et al.Hapten-Grafted Programmed Probe as a Corecognition Element for a Competitive Immunosensor to Detect Acetamiprid Residue in Agricultural Products[J].Journal of agricultural and food chemistry, 2018, 66(29):7815-21 [34]PéREZ-FERNáNDEZ B, MERCADER J V, CHECA-ORREGO B I, et al.A monoclonal antibody-based immunosensor for the electrochemical detection of imidacloprid pesticide[J].The Analyst, 2019, 144(9):2936-41 [35]DING S, GU Z, YAN R, et al.A novel mode of DNA assembly at electrode and its application to protein quantification [J]. Analytica chimica acta, 2018, 1029: 24-9. DOI: 10.1016/j.aca.2018.04.073. [36]LI F, GUO Y, WANG X, et al.Multiplexed aptasensor based on metal ions labels for simultaneous detection of multiple antibiotic residues in milk [J]. Biosensors & bioelectronics, 2018, 115: 7-13. DOI: 10.1016/j.bios.2018.04.024. [37]MADIANOS L, TSEKENIS G, SKOTADIS E, et al.A highly sensitive impedimetric aptasensor for the selective detection of acetamiprid and atrazine based on microwires formed by platinum nanoparticles [J]. Biosensors & bioelectronics, 2018, 101: 268-74. DOI: 10.1016/j.bios.2017.10.034. [38]QIAO X, XIA F, TIAN D, et al.Ultrasensitive " signal-on" electrochemical aptasensor for assay of acetamiprid residues based on copper-centered metal-organic frameworks [J]. Analytica chimica acta, 2019, 1050: 51-9. DOI: 10.1016/j.aca.2018.11.004. [39]YI J, LIU Z, LIU J, et al.A label-free electrochemical aptasensor based on 3D porous CS/rGO/GCE for acetamiprid residue detection [J]. Biosensors & bioelectronics, 2020, 148: 111827. DOI: 10.1016/j.bios.2019.111827. [40]DAMBORSKy P, ?VITEL J, KATRLíK J.Optical biosensors[J].Essays in Biochemistry, 2016, 60(1):91-100 [41]MA F, LI Y, TANG B, et al.Fluorescent Biosensors Based on Single-Molecule Counting[J].Accounts of chemical research, 2016, 49(9):1722-30 [42]YAN X, LI H, SU X.Review of optical sensors for pesticides [J]. TrAC Trends in Analytical Chemistry, 2018, 103: 1-20. DOI: 10.1016/j.trac.2018.03.004. [43]LI H, JIN R, KONG D, et al.Switchable fluorescence immunoassay using gold nanoclusters anchored cobalt oxyhydroxide composite for sensitive detection of imidacloprid [J]. Sensors and Actuators B: Chemical, 2019, 283: 207-14. DOI: 10.1016/j.snb.2018.12.026. [44]GUO Y, ZOU R, SI F, et al.A sensitive immunoassay based on fluorescence resonance energy transfer from up-converting nanoparticles and graphene oxide for one-step detection of imidacloprid [J]. Food chemistry, 2021, 335: 127609. DOI: 10.1016/j.foodchem.2020.127609. [45]BAHREYNI A, YAZDIAN-ROBATI R, RAMEZANI M, et al.Fluorometric aptasensing of the neonicotinoid insecticide acetamiprid by using multiple complementary strands and gold nanoparticles[J].Mikrochimica acta, 2018, 185(5):272- [46]YAN C, SHI G, CHEN J.Fluorescent Detection of Two Pesticides Based on CRISPR-Cas12a and Its Application for the Construction of Four Molecular Logic Gates[J].Journal of agricultural and food chemistry, 2022, 70(39):12700-7 [47]朱琪琦.纳米酶基比色传感器在疾病标识物检测中的应用[D].石河子: 石河子大学, 2022: 000854. [48]韩高寰, 李静, 张冰.基于纳米材料的比色适配体传感器的应用[J].化学试剂, 2022, 44(06):801-9 [49]LIU J, GUAN Z, LV Z, et al.Improving sensitivity of gold nanoparticle based fluorescence quenching and colorimetric aptasensor by using water resuspended gold nanoparticle [J]. Biosensors & bioelectronics, 2014, 52: 265-70. DOI: 10.1016/j.bios.2013.08.059. [50]GELETA G S.A colorimetric aptasensor based on gold nanoparticles for detection of microbial toxins: an alternative approach to conventional methods[J].Analytical and bioanalytical chemistry, 2022, 414(24):7103-22 [51]SHI H, ZHAO G, LIU M, et al.Aptamer-based colorimetric sensing of acetamiprid in soil samples: sensitivity, selectivity and mechanism [J]. Journal of hazardous materials, 2013, 260: 754-61. DOI: 10.1016/j.jhazmat.2013.06.031. [52]YANG L, WANG X, SUN H, et al.A syringe-aided apta-nanosensing method for colorimetric determination of acetamiprid [J]. Analytica chimica acta, 2021, 1150: 238118. DOI: 10.1016/j.aca.2020.11.050. [53]ZHAO T, LIANG X, GUO X, et al.Smartphone-based colorimetric sensor array using gold nanoparticles for rapid distinguishment of multiple pesticides in real samples[J].Food chemistry, 2023, 404(Pt B):134768- [54]BABAZADEH S, MOGHADDAM P A, KESHIPOUR S, et al.Colorimetric sensing of imidacloprid in cucumber fruits using a graphene quantum dotAu (III) chemosensor[J].Scientific reports, 2020, 10(1):14327- [55]BABAMIRI B, BAHARI D, SALIMI A.Highly sensitive bioaffinity electrochemiluminescence sensors: Recent advances and future directions [J]. Biosensors & bioelectronics, 2019, 142: 111530. DOI: 10.1016/j.bios.2019.111530. [56]GUO Y, YANG F, YAO Y, et al.Novel Au-tetrahedral aptamer nanostructure for the electrochemiluminescence detection of acetamiprid [J]. Journal of hazardous materials, 2021, 401: 123794. DOI: 10.1016/j.jhazmat.2020.123794. [57]TANG F, HUA Q, WANG X, et al.A novel electrochemiluminescence sensor based on a molecular imprinting technique and UCNPs@ZIF-8 nanocomposites for sensitive determination of imidacloprid[J].The Analyst, 2022, 147(17):3917-23 [58]YANG J, DENG C, ZHONG W, et al.Electrochemical activation of oxygen vacancy-rich TiO(2)@MXene as high-performance electrochemical sensing platform for detecting imidacloprid in fruits and vegetables[J].Mikrochimica acta, 2023, 190(4):146- [59]HAN J, YU Y, WANG G, et al.Ultrasensitive electrochemiluminescence aptasensor based on ABEI reduced silver nanoparticles for the detection of profenofos [J]. The Science of the total environment, 2022, 844: 157184. DOI: 10.1016/j.scitotenv.2022.157184. [60]LU Z, DAI S, LIU T, et al.Machine learning-assisted Te-CdS@Mn(3)O(4) nano-enzyme induced self-enhanced molecularly imprinted ratiometric electrochemiluminescence sensor with smartphone for portable and visual monitoring of 2, 4-D [J]. Biosensors & bioelectronics, 2023, 222: 114996. DOI: 10.1016/j.bios.2022.114996. [61]AN D, SHEN Y, WEN J, et al.Molybdenum Nanoscrews: A Novel Non-coinage-Metal Substrate for Surface-Enhanced Raman Scattering[J].Nano-micro letters, 2017, 9(1):2- [62]LI C, WANG H, LUO Y, et al.A novel gold nanosol SERS quantitative analysis method for trace Na(+) based on carbon dot catalysis [J]. Food chemistry, 2019, 289: 531-6. DOI: 10.1016/j.foodchem.2019.03.032. [63]ONG T T X, BLANCH E W, JONES O A H.Surface Enhanced Raman Spectroscopy in environmental analysis, monitoring and assessment [J]. The Science of the total environment, 2020, 720: 137601. DOI: 10.1016/j.scitotenv.2020.137601. [64]KUTSANEDZIE F Y H, AGYEKUM A A, ANNAVARAM V, et al.Signal-enhanced SERS-sensors of CAR-PLS and GA-PLS coupled AgNPs for ochratoxin A and aflatoxin B1 detection [J]. Food chemistry, 2020, 315: 126231. DOI: 10.1016/j.foodchem.2020.126231. [65]XU Y, KUTSANEDZIE F Y H, HASSAN M, et al.Mesoporous silica supported orderly-spaced gold nanoparticles SERS-based sensor for pesticides detection in food [J]. Food chemistry, 2020, 315: 126300. DOI: 10.1016/j.foodchem.2020.126300. [66]ZHOU J, WANG D, YANG H, et al.Specific detection of acetamiprid with aptamer based on flexible and adhesive SERS membrane [J]. Spectrochimica acta Part A, Molecular and biomolecular spectroscopy, 2022, 270: 120801. DOI: 10.1016/j.saa.2021.120801. [67]ZHU C, DU D, EYCHMüLLER A, et al.Engineering Ordered and Nonordered Porous Noble Metal Nanostructures: Synthesis,Assembly,and Their Applications in Electrochemistry[J].Chemical reviews, 2015, 115(16):8896-943 [68]WANG S-H, LO S-C, TUNG Y-J, et al.Multichannel nanoplasmonic platform for imidacloprid and fipronil residues rapid screen detection [J]. Biosensors and Bioelectronics, 2020, 170. DOI: 10.1016/j.bios.2020.112677. [69]CHANG T-W, WANG S-H, CHIN I-S, et al.Biomimetic affinity sensor for the ultrasensitive detection of neonicotinoids [J]. Biosensors and Bioelectronics, 2023, 239. DOI: 10.1016/j.bios.2023.115630. [70]CODLING G, AL NAGGAR Y, GIESY J P, et al.Concentrations of neonicotinoid insecticides in honey, pollen and honey bees (Apis mellifera L.) in central Saskatchewan, Canada [J]. Chemosphere, 2016, 144: 2321-8. DOI: 10.1016/j.chemosphere.2015.10.135.