副干酪乳杆菌FZU103对小鼠酒精性肝损伤的防控作用

摘要/Abstract

摘要： 摘要：目的：探究副干酪乳杆菌FZU103（Lactobacillus paracasei-FZU103，LP-FZU103）对酒精性肝损伤（alcoholic liver injury，ALI）的防控作用。方法：选取36只SPF级ICR小鼠，随机分为空白组、模型组、实验组（LP-FZU103干预），实验6周后进行体重及脏器系数、血清及肝脏生化、肝组织病理学及炎症因子、肝功能相关基因转录和肠道菌群组成的测定。结果：与模型组相比，LP-FZU103干预可改善酒精性肝损伤小鼠的脏器系数和肝组织病理损伤程度，显著降低血清中总胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）和低密度脂蛋白胆固醇（low density lipoprotein cholesterol，LDL-C）的浓度以及谷丙转氨酶（alanine aminotransferase，ALT）和谷草转氨酶（aspartate aminotransferase，AST）的活性，提高血清高密度脂蛋白胆固醇（high density lipoprotein cholesterol，HDL-C）的浓度；显著提高肝脏中过氧化氢酶（catalase，CAT）和超氧化物歧化酶（superoxide dismutase，SOD）的活性以及谷胱甘肽（glutathione，GSH）的含量，降低肝脏中丙二醛（malondialdehyde，MDA）含量和白细胞介素-6（interleukin-6，IL-6）、干扰素-γ（interferon-γ，IFN-γ）水平；显著上调脂质代谢相关基因LDLR 及下调ACC1、HMGCR和CD36的mRNA水平；显著增加了小鼠肠道中约氏乳杆菌（Lactobacillus johnsonii）、罗伊氏乳杆菌（Lactobacillus reuteri）、副干酪乳杆菌（Lactobacillus paracasei）等有益细菌的相对丰度。结论：LP-FZU103的干预可一定程度上防控小鼠酒精性肝损伤的发生，这与其改善的肠道菌群及肝脏代谢功能密切相关。

关键词: 副干酪乳杆菌, 酒精性肝损伤, 氧化应激, 肝功能基因, 肠道菌群

Abstract: Abstract: Objective: To explore the prevention and control effect of Lactobacillus paracasei-FZU103 (LP-FZU103) on alcoholic liver injury (ALI). Methods: 36 SPF grade ICR mice were randomly divided into control group, model group and experimental group (LP-FZU103 intervention). After 6 weeks of experiment, body weight, organ coefficient, serum and liver biochemistry, liver histopathology and inflammatory factors, transcription of liver function related genes and intestinal flora composition were measured. Result: Compared with the model group, the intervention of LP-FZU103 improved the organ coefficients and pathological damage degree of liver tissue in mice with alcohol-induced liver injury, and significantly reduced serum total cholesterol (TC) and triglyceride (TG). The concentrations of TG, LDL-C and alanine aminotransferase, The activity of ALT and aspartate aminotransferase (AST) increased the concentration of serum high density lipoprotein cholesterol (HDL-C). Significantly increased the activities of catalase (CAT) and superoxide dismutase (SOD) and the content of glutathione (GSH) in liver, and decreased malondialdehyde (dehyde) in liver. MDA content and interleukin-6 (IL-6), interferon-γ (IFN-γ) levels; Significantly up-regulated the mRNA levels of lipid metabolism-related genes LDLR and down-regulated ACC1, HMGCR and CD36; The relative abundance of beneficial bacteria such as Lactobacillus johnsonii, Lactobacillus reuteri and Lactobacillus paracasei in the gut of mice was significantly increased. Conclusion:.The intervention of LP-FZU103 can prevent and control the occurrence of alcoholic liver injury in mice to a certain extent, which is closely related to the improved intestinal flora and liver metabolic function.

Key words: Lactobacillus paracasei, alcoholic liver injury, oxidative stress, liver function gene, intestinal flora

中图分类号:

TS201.4

梁梓华李嘉仪谢林惠杨梓翊尤适泽吴超李文龙陈有挺艾连中倪莉吕旭聪. 副干酪乳杆菌FZU103对小鼠酒精性肝损伤的防控作用[J]. 食品科学, 2024, 45(7): 0-0.

Ni Li Lv xucong. Prevention and Control Effect of Lactobacillus paracasei FZU103 on Alcoholic Liver Injury in Mice[J]. FOOD SCIENCE, 2024, 45(7): 0-0.

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