FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (18 ): 131-134.doi: 10.7506/spkx1002-6300-200918026

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Application of Response Surface Methodology to Optimize Extraction Conditions of Polysaccharides from Herba Moslae

LI Jing-en,NIE Shao-ping*,YANG Chao,QIU Zeng-hui,XIE Ming-yong,LI Chang,WAN Yin   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Received:2009-04-21 Online:2009-09-15 Published:2010-12-29
  • Contact: NIE Shao-ping E-mail:spnie@ncu.edu.cn

Abstract:

The hot water extraction procedure of Herba Moslae polysaccharides was studied. On the basis of single-factor experiments, a three-factor, three-level Box-Behnken design combining with response surface methodology (RSM) was employed to optimize three crucial extraction conditions for maximizing yield of polysaccharides from Herba Moslae. A regression model was established for yield of polysaccharides as a function of the three extraction conditions. The analysis for this model by SAS V8.0 software indicated that the optimal extraction temperature, time and ratio of water to material were 87.0 ℃, 4.0 h and 15.4:1 (ml/g), respectively. Under these conditions, the predicted value of yield of polysaccharides from Herba moslae was 3.38%, in basic agreement with the actual value of 3.41%.

Key words: polysaccharides from Herba Moslae, extraction, response surface methodology

CLC Number: