FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (18 ): 159-162.doi: 10.7506/spkx1002-6300-200918033

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Aqueous Two-phase Extraction of Cold-adapted α-Amylase from Fermentation Culture Supernatant of Pseudoalteromonas arctic GS230

FANG Yao-wei1,LIU Shu1,LU Ming-sheng1,XU Wei-feng2,WANG Shu-jun1,*, CHEN Li1,JIAO Yu-liang   

  1. 1. Jiangsu Key Laboratory of Marine Biotechnology, School of Marine Science and Technology, Huaihai Institute of Technology,
    Lianyungang 222005, China ;2. Jiangsu Agro-product Quality Text Center, Nanjing 210036, China
  • Received:2009-06-27 Online:2009-09-15 Published:2010-12-29
  • Contact: WANG Shu-jun E-mail:shujunwang@163.com

Abstract:

An aqueous two-phase system consisting of polyethylene glycol and phosphate was applied to extract cold-adapted α -amylase from fermentation culture supernatant of Pseudoalteromonas arctic GS230. The effects of phase composition, molecular weight of PEG and pH on enzyme partitioning and selective enrichment were studied, and the extraction procedure established was verified by linear scale-up experiments. A maximum purification fold of 4.8 and enzyme activity recovery of 87% were obtained when an aqueous 15% PEG-15% phosphate system at pH 5.0 was used for the extraction.

Key words: aqueous two-phase system (ATPS), extraction, cold-adapted α-amylase

CLC Number: