Abstract:

A HPLC method was established to determine polyphenolic compounds in Flammulina velutipe fruitbody. The chromatographic separation was achieved on an Agilent Zorbax SB-C18 (4.6 mm × 250 mm, 5 μm) analytical column using a mobile phase consisting of a mixture of A: acetonitrile and B: 0.4% acetic acid (0–40 min: 5% A and 95% B; 40–45 min: 25% A and 75% B; 45–50 min: 35% A and 65% B) at a flow rate of 1.0 ml/min and 30 ℃ column temperature , and the analytes were detected using a VWD detector at 280 nm wavelength. Rutin and quercetin both were found in fresh Flammulina velutipe fruitbody, while quercetin, together with syringic acid was detected in dried samples.

Key words: Flammulina velutipe, rutin, quercetin, syringic acid, RP-HPLC