Abstract:

In order to optimize ultrasonic extraction of polysaccharides in Lonicera japonica, a mathematical regression model for predicting extraction yield of polysaccharides as a function of ratio of independent variables, including water/material ratio, extraction temperature and extraction time was established by fitting Box-Benhnken central composite design experimental results and interactive effects of the independent variables on extraction yield of polysaccharides were investigated by response surface methodology based on the analysis of single-factor experiments. The optimal protocol for polysaccharides extraction from Lonicera japonica was based on twice extraction for 43 min at 75 ℃ with a ratio of water to dry material of 23:1, which yielded an extraction yield of 3.26%. Results indicated that ultrasonic method is characteristic of time-saving compared with other traditional methods.

Key words: Lonicera japonica, ultrasonic extraction, polysaccharide, process, response surface analysis