in vitro Anti-Lipid Peroxidation and Red Blood Cell Protecting Effects of Fermentation Broth Extract Produced by Endophytic Chaetomium globosum from Eucommia ulmoides

doi:10.7506/spkx1002-6300-200921083

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (21): 355-359.doi: 10.7506/spkx1002-6300-200921083

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in vitro Anti-Lipid Peroxidation and Red Blood Cell Protecting Effects of Fermentation Broth Extract Produced by Endophytic Chaetomium globosum from Eucommia ulmoides

XIE Hui，CHEN Shuang-lin*，YAO Hui-qin，WU Ming-qian，WANG Mei-xia

College of Life Sciences, Nanjing Normal University, Nanjing 210046, China

Received:2008-11-28 Revised:2009-07-20 Online:2009-11-01 Published:2010-12-29
Contact: CHEN Shuang-lin E-mail:chenshuanglin@njnu.edu.cn

Abstract

Abstract:

A strain of endophytic Chaetomium globosum with high antixoxidant activity was isolated from Eucommia ulmoide, and its fermentation broth was extracted for the evaluation of in vitro inhibitory effects on automatic and H2O2-induced lipid peroxidation and hemolysis of red blood cells (RBCs). Results showed that the extract exhibited a dose-dependent inhibitory effect against lipid auto-peroxidation in mouse heart and spleen tissues and H2O2-induced lipid peroxidation in liver tissue, with inhibition ratios of 71.55%, 71.68% and 92.50% at 1 mg/ml, respectively. Moreover, the extract also inhibited 68.48% of autooxidative hemolysis of RBCs at 0.3 mg/ml and 96.12% of H2O2-induced hemolysis of RBCs at 0.75 mg/ml, and the inhibitory effect against H2O2-induced hemolysis of RBCs exhibited an increase with increasing concentration. In conclusion, this extract has significant in vitro anti-lipid peroxidation activity and protective effects on red blood cells resulting from decrease in oxidative hemolysis.

Key words: Eucommia ulmoides, Chaetomium globosum, anti-lipid peroxidation activity

CLC Number:

TS201.3

XIE Hui，CHEN Shuang-lin*，YAO Hui-qin，WU Ming-qian，WANG Mei-xia. in vitro Anti-Lipid Peroxidation and Red Blood Cell Protecting Effects of Fermentation Broth Extract Produced by Endophytic Chaetomium globosum from Eucommia ulmoides[J]. FOOD SCIENCE, 2009, 30(21): 355-359.

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in vitro Anti-Lipid Peroxidation and Red Blood Cell Protecting Effects of Fermentation Broth Extract Produced by Endophytic Chaetomium globosum from Eucommia ulmoides

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