FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22): 157-161.doi: 10.7506/spkx1002-6300-200922034

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Optimization of Alkaloid Extraction from Lotus Leaves by Response Surface Method

XIAO Juan,SUN Zhi-da*,XIE Bi-jun,YANG Er-ning   

  1. (College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
  • Received:2008-12-23 Revised:2009-07-11 Online:2009-11-15 Published:2010-12-29
  • Contact: SUN Zhi-da*, E-mail:sunzhida@mail.hzau.edu.cn

Abstract:

Optimal condition for alkaloid extraction from lotus leaves was investigated through response surface method based on single factor experiments to study the effect of extraction temper ature, extraction time, alcohol concentration and liquidmaterial ratio on extraction rate. The optimal extraction condition was 84 ℃ of extraction temperature, 2.01 h of extraction time, 90.65% of alcohol, and 29.74:1 (ml/g) of liquid-material ratio. Results indicated that alkaloid extraction from lotus leaves was 0.858% under this optimal condition. A linear was observed in the range of 6.40-32.00 μg/ml (r = 0.992). The content of nuciferine in alkaloids from lotus leaves was 7.952%.

Key words: lotus leaf, alkaloid, nuciferine, response surface method, HPLC

CLC Number: