Preparation and Optimization of Immunoaffinity Column for Ciprofloxacin

doi:10.7506/spkx1002-6300-200922047

Abstract

Abstract:

In order to prepare optimal immunoaffinity column (IAC) for ciprofloxacin, elution parameters and separation efficiency were systematically analyzed. The excellent separation performance was observed using 4 ml of 50% methanol in PBS as elutent. Column capacity remained 25% of original capacity after 5 cycles of use at the optimized condition. Recovery rate determination was conducted using high performance liquid chromatography with a fluorescence detector (HPLC-FLD) under the gradient elution through 0.05% formic acid aqueous solution-methanol. The HPLC-FLD method exhibited an excellent linear in the range of 10－1000 ng/ml with a correlation coefficient of 0.99. Recovery rate of ciprofloxacin using IAC was 74.38%－ 105.552%.

Key words: quinolones, ciprofloxacin, immunoaffinity column (IAC)

CLC Number:

TS207.5

LIU Li-qiang，LI Yin，PENG Chi-fang，CHEN Wei，XU Chuan-lai*. Preparation and Optimization of Immunoaffinity Column for Ciprofloxacin[J]. FOOD SCIENCE, 2009, 30(22 ): 209-212.

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Preparation and Optimization of Immunoaffinity Column for Ciprofloxacin

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