Abstract:

Chondroitin sulfate (ChS) was extracted from shark fin cartilage, and the effects of alkaline concentration (x1), extraction temperature (x2) and time (x3) on the yield were studied. By conducting response surface analysis, the optimum extraction conditions were obtained, and a multiple regression model was set up between the extraction conditions and the yield of ChS, then the effect of ultrasonic time on the yield and purity of ChS was studied. The mathematical model between the extraction conditions and yield of shark ChS as follows: ChS(%)=14.56－0.34x2－0.34x1x2－0.82x12－1.45x22－0.47x32. The extraction temperature significantly affected the yield of ChS, followed by the alkaline concentration. According to the response surface analysis, the optimal extraction conditions were as follows: temperature 35 ℃, time 3 h, alkaline concentration 4% with the yield of ChS 14.50%. The yield and purity of ChS increased to 18.07% and 91.58% respectively, if shark fin cartilage was processed by ultrasound for 20 min prior to alkaline extraction.

Key words: shark fin cartilage, chondroitin sulfate, alkaline extraction, response surface method, ultrasonic wave