Analysis of Fatty Acids in Spirulina by Gas Chromatography-Mass Spectrometry

doi:10.7506/spkx1002-6300-200922065

Abstract

Abstract:

A gas chromatography-mass spectrometry (GC-MS) method was established to identify compositions of fatty acids and determine the content ofγ-linolenic acid in Spirulina. The compositions of fatty acids in Spirulina were identified through MS and γ -linolenic acid was quantitatively determined by a normalization method of total ion chromatograph. Qualitative analysis results revealed that 10 unsaturated fatty acids were detected and the content of unsaturated fatty acids was 46.774%; meanwhile, the content ofγ-linolenic acid was 26.702%. The determination ofγ-linolenic acid using GC-MS was expressed to be Y = 15843X－533217 with a correlation coefficient of 0.9986. An excellent linear range forγ-linolenic acid was 50.0 －5000.0 μg/ml. Total content of linolenic acid in Spirulina was 396 mg/100 g andγ-linolenic acid was 393 mg/100 g.

Key words: Spirulina, fatty acid, gas chromatography-mass spectrometry (GC-MS)

CLC Number:

O657.71

XU Hua，PAN Can-ping*. Analysis of Fatty Acids in Spirulina by Gas Chromatography-Mass Spectrometry[J]. FOOD SCIENCE, 2009, 30(22): 280-282.

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Analysis of Fatty Acids in Spirulina by Gas Chromatography-Mass Spectrometry

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