FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (23 ): 343-346.doi: 10.7506/spkx1002-6300-200923078

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Isolation and Purification of Oligosaccharides from Soybean Molasses through Fermentation

CUI Xi-qing1,2,LIU Chang1,2,DONG Yin-mao2,HE Cong-fen2,WANG Chang-tao2,*   

  1. 1.Collge of Life Science, Northeast Agricultural University, Harbin 150030, China; 2. Beijing Key Laboratory of Plant Resources
    Research and Development, Beijing Technology and Business University, Beijing 100048, China
  • Received:2009-09-05 Online:2009-12-01 Published:2010-12-29
  • Contact: WANG Chang-tao E-mail:wangct@th.btbu.edu.cn

Abstract:

One strain of yeast that has the function of high consumption to sucrose, and high retention rate to stachyose and raffinose was screened from three stains of yeast, one strain of Lactobacillus, one strain of Aspergillus. The optimal condition for the fermentation of soybean molasses was initial pH 5, 2% inoculation amount, 1:8 of dilution for soybean molasses, 180 r/min shaking and fermentation at 28 ℃ for 12 h. The retention rates in hydrolysates of soybean molasses were 8.76% for sucrose, 99.61% for raffinose and 95.72% for stachyose under this optimal fermentation condition.

Key words: oligosaccharide, soybean molasses, fermentation, retention rate

CLC Number: