Screening and Identification of 1- Deoxynojirimycin-Producing Bacteria Strain

doi:10.7506/spkx1002-6300-200923082

Abstract

Abstract:

In present research, 1-deoxynojirimycin-producing bacteria strain was isolated from the soil of mulberry field and screened by glucoamylase agar plate. Seven strains indicating blue in the plate were isolated from the total 263 microorganisms. A new 1-deoxynojirimycin-producing strain of SL-429 was obtained by using the RP-HPLC coupled with UV detection (UVRP- HPLC. The yield of 1-deoxynojirimycin was over 0.017mg/ml under the normal fermentation at 27℃ with the medium comprising 0.05% KCl, 0.05%MgSO4·7 H2O, 0.2%NaNO3, 0.5%NaCl, 0.35%CaCO3, 4% soluble starch, 1% soybean meal and 1% tryptose. This 1-deoxynojirimycin-producing strain was identified as Streptomyces lavendulae based on its morphological, physiological and biochemical characters.

Key words: 1-deoxynojirimycin, Streptomyces lavendulae, screening, reverse high performance liquid chromatography

CLC Number:

Q93.335

ZHOU Le-chun，WEI Zhao-jun*，ZHANG Hai-xiang，JIANG Lei. Screening and Identification of 1- Deoxynojirimycin-Producing Bacteria Strain[J]. FOOD SCIENCE, 2009, 30(23 ): 361-364.

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Screening and Identification of 1- Deoxynojirimycin-Producing Bacteria Strain

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