Abstract:

A rapid high performance liquid chromatographic (HPLC) method was presented for determining amygdalin in Semen Armeniacae Amarum, bitter almond and Semen Persicae. The chromatographic separation was performed on a Shim-pack VPODS column (150 mm × 4.6 mm, 5 μm) with a mobile phase composed of methanol and water (20:80, V/V) at a flow rate of 1 mL/min, the column temperature was set at 40 ℃, and the analyte was detected at 215 nm wavelength. Results showed that mygdalin was separated well and analyzed effectively by the method. There was a good linearity between relative peak area (X) and amygdalin concentration (Y), and the linear regression equation was Y ＝ 4.86244 × 10-6X －0.0129273. Methodological investigations demonstrated the advantages of this method, including good repeatability and high precision, which benefit its application to rapid online determination of amygdalin in dried nuts.

Key words: Semen Armeniacae Amarum, Semen Persicae, amygdalin, HPLC