FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (2): 138-140.doi: 10.7506/spkx1002-6300-201002034

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Rapid HPLC Determination of Amygdalin in Dried Nuts Grown in Xinjiang

ZHANG Fan1,2,LI Zhen1,HAMULATI Wufuer2,GAO Fu-chun1   

  1. 1. Chinese Medicine College, Xinjiang Medical University, Urumqi 830054, China;
    2. Pharmaceutical Post-doctoral Station of Xinjiang Medical University, Urumqi 830054, China
  • Received:2009-02-27 Revised:2009-08-07 Online:2010-01-15 Published:2010-12-29
  • Contact: ZHANG Fan1,2


A rapid high performance liquid chromatographic (HPLC) method was presented for determining amygdalin in Semen Armeniacae Amarum, bitter almond and Semen Persicae. The chromatographic separation was performed on a Shim-pack VPODS column (150 mm × 4.6 mm, 5 μm) with a mobile phase composed of methanol and water (20:80, V/V) at a flow rate of 1 mL/min, the column temperature was set at 40 ℃, and the analyte was detected at 215 nm wavelength. Results showed that mygdalin was separated well and analyzed effectively by the method. There was a good linearity between relative peak area (X) and amygdalin concentration (Y), and the linear regression equation was Y = 4.86244 × 10-6X -0.0129273. Methodological investigations demonstrated the advantages of this method, including good repeatability and high precision, which benefit its application to rapid online determination of amygdalin in dried nuts.

Key words: Semen Armeniacae Amarum, Semen Persicae, amygdalin, HPLC

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