Abstract:

Recombinant xylanase PTX2 was prepared in a 15 L fermentor through high-density fermentation. After methanol induction at 28 ℃ for 96 h, the highest cell density and the maximal xylanase activity were achieved to 78 g/L and 560 U/mL, respectively. Diet supplemented with 500 U/kg of unprocessed PTX2 could result in an increase of average body weight gain of weaned piglets by 16.9%, an obvious decrease in intake/body weight gain ratio, and an increasing trend of apparent digestibility of crude protein (CP), ash, calcium and crude fiber (CF). Therefore, the potential benefit of PTX2 in feeding weaned piglets demonstrates an attractive application value and industrial development of this enzyme.  

Key words: xylanase, high-density fermentation, weaned piglets, growth performance, apparent digestibility