Screening of Tow Xylanase-producing Fungus Strains and Study on Enzymological Properties of Produced Xylanases

doi:10.7506/spkx1002-6630-200901038

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (1): 159-163.doi: 10.7506/spkx1002-6630-200901038

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Screening of Tow Xylanase-producing Fungus Strains and Study on Enzymological Properties of Produced Xylanases

WU Ping，SHI Jun，LUO Bing-guo

(College of Life Science, Anhui Science and Technology University, Fengyang 233100, China)

Received:2007-12-04 Revised:2008-07-28 Online:2009-01-01 Published:2010-12-29
Contact: WU Ping E-mail:wupinggu@126.com

Abstract

Abstract:

Two strains of fungi (C4 and G1) producing high activity xylanase were isolated by soil dilution coating method and through primary and repeated screening. Furthermore, the optimum carbon source for their growth and enzymological properties of xylanases produced by them were studied. The results showed that the best carbon source for strain C4 producing xylanase is the mixture of corncob and bran. The xylanse activity produced by strain G4 reaches the peak after cultivation for 24h, 6582. 29 U/ml. The optimum reaction temperature of the xylanse is 40 ℃ and it is relatively stable below 50 ℃. Its optimum reaction pH is 5.0 and it shows good pH stability; The best carbon source for strain G1 producing xylanase is corncob. The xylanase activity produced by strain G1 reaches the peak after 96 hours of cultivation, 6301.04 U/ml. The optimum reaction temperature of xylanase produced by strain G1 is 40 ℃ and it is stable below 40 ℃. The optimum reaction pH is 5.0, but its stability is less than that of xylanase produced by strain G4.

Key words: fungus, xylanase, enzymological properties, screening

CLC Number:

Q814.1

WU Ping，SHI Jun，LUO Bing-guo. Screening of Tow Xylanase-producing Fungus Strains and Study on Enzymological Properties of Produced Xylanases[J]. FOOD SCIENCE, 2009, 30(1): 159-163.

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Screening of Tow Xylanase-producing Fungus Strains and Study on Enzymological Properties of Produced Xylanases

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